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논문 기본 정보

자료유형
학술저널
저자정보
Hyejin Cho (Chung-Ang University) Sullim Lee (Gachon University) Myoung-Sook Shin (Gachon University) Joohwan Lee (Healthy Spoon Research Institute) Sanghyun Lee (Chung-Ang University)
저널정보
한국생약학회 생약학회지(Korean Journal of Pharmacognosy) 생약학회지 제54권 제1호
발행연도
2023.3
수록면
38 - 43 (6page)

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A simple and accurate assay was developed for the quantitative analysis of 1-deoxynojirimycin (1-DNJ) derived from the silkworm (Bombyx mori). Normal-phase high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) and a hydrophilic interaction liquid chromatography column was used. Various parameters were applied to optimize the analysis method. The limits of detection and quantification of 1-DNJ were 2.97 × 10<SUP>-3</SUP> and 9.00 × 10<SUP>-3</SUP> mg/mL, respectively. The calibration curve showed good linearity results. The concentration range and the r² value were 0.0625-1.0 mg/mL and 0.9997, respectively. The accuracy test demonstrated a significantly high recovery rate (89.95-103.22%). The relative standard deviation was ≤ 1.00%. Thus, a method for the accurate identification and quantitative analysis of 1-DNJ in silkworms was developed. Moreover, in this procedure, the process of derivatization of 1-DNJ, which was required in previous experiments, could be eliminated. This technique may be actively utilized for the development of pharmaceuticals and health functional foods using 1-DNJ.

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Abstract
Materials and Methods
Results and Discussion
Conclusions
References

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