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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.2
- 수록면
- 141 - 148 (8page)
이용수
초록· 키워드
Oriental melon (Cucumis melo L. var. makuwa) is one of six subspecies of melon and is cultivated widely in East Asia, including China, Japan, and Korea. Although orien-tal melon is economically valuable in Asia and is genet-ically distinct from other subspecies, few reports of ge-nome-scale research on oriental melon have been pub-lished. We generated 30.5 and 36.8 Gb of raw RNA se-quence data from the female and male flowers, leaves, roots, and fruit of two oriental melon varieties, Korean landrace (KM) and Breeding line of NongWoo Bio Co. (NW), respectively. From the raw reads, 64,998 transcripts from KM and 100,234 transcripts from NW were de novo assembled. The assembled transcripts were used to identify molecular markers (e.g., single-nucleotide polymorphisms and simple sequence repeats), detect tissue-specific expressed genes, and construct a genetic linkage map. In total, 234 single-nucleotide poly-morphisms and 25 simple sequence repeats were screened from 7,871 and 8,052 candidates, respectively, between the KM and NW varieties and used for construc-tion of a genetic map with 94 F2 population specimens. The genetic linkage map consisted of 12 linkage groups, and 248 markers were assigned. These transcriptome and molecular marker data provide information useful for molecular breeding of oriental melon and further com-parative studies of the Cucurbitaceae family.
#genetic linkage map
#Korean melon
#simple sequence repeat
#single-nucleotide polymorphism
#transcriptome analysis
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