Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling

Ning Chen; Yuli Wang; Junchi Ma; Yifei Du; Jing Miao

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자료유형: 학술저널

저자정보: Ning Chen (Nanjing Medical University) Yuli Wang (Nanjing Medical University) Junchi Ma (Nanjing Medical University) Yifei Du (Nanjing Medical University) Jing Miao (Nanjing Medical University)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제39권 제3호

발행연도: 2016.3

수록면: 186 - 194 (9page)

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Epidemiological evidence suggests that bone is especial-ly sensitive to oxidative stress, causing bone loss in the elderly. Previous studies indicated that human amnion-derived mesenchymal stem cells (HAMSCs), obtained from human amniotic membranes, exerted osteoprotective effects in vivo. However, the potential of HAMSCs as seed cells against oxidative stress-mediated dysfunction is unknown. In this study, we systemically investigated their antioxidative and osteogenic effects in vitro. Here, we demonstrated that HAMSCs signi?cantly promoted the proliferation and osteoblastic differentiation of H2O2-induced human bone marrow mesenchymal stem cells (HBMSCs), and down-regulated the reactive oxygen species (ROS) level. Further, our results suggest that activation of the ERK1/2 MAPK signal transduction pathway is essential for both HAMSCs-mediated osteogenic and protective effects against oxidative stress-induced dysfunction in HBMSCs. U0126, a highly selective inhibitor of extracellular ERK1/2 MAPK signaling, significantly suppressed the antioxidative and osteogenic effects in HAMSCs. In conclusion, by modulating HBMSCs, HAMSCs show a strong potential in treating oxidative stress- mediated bone deficiency.

#ERK1/2 MAPK signal transduction pathway #human amnion mesenchymal stem cells (HAMSCs) #human bone marrow mesenchymal stem cells (HBMSCs) #oxidative stress #reactive oxygen species (ROS)

참고문헌 (61)

참고문헌 신청

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Chen, Y. J. / 2012 / The effects of acellular amniotic membrane matrix on osteogenic differentiation and ERK1/2 signaling in human dental apical papilla cells / Biomaterials 33:455~463

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