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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 497 - 503 (7page)
- DOI
- 10.4014/mbl.1606.06009
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초록· 키워드
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This study aimed to optimize the culture conditions of recombinant Escherichia coli expressing otsBA using crude glycerol for the enhanced production of trehalose. The effects of culture temperature and isopropyl β-D-1-thiogalactopyranoside (IPTG)-induction were investigated. Trehalose production and cell growth were highest when cells were cultured at 37℃ and induced with IPTG. The concentrations of IPTG, validamycin A, and NaCl were optimized using Box-Behnken design. Statistical analyses of the experimental data revealed that the concentrations of IPTG and NaCl had significant effects on trehalose production, but that of validamycin A did not. Contour plot analysis and model calculation showed that the highest amount of trehalose could be produced at 298 mM NaCl and 0.1 mM IPTG. Under these optimal conditions, the optical density at 600 nm and trehalose production were 5.4 ± 0.2 and 304 ± 15 mg/l, respectively.
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참고문헌 (28)
참고문헌 신청
Cabiscol E / 2000 / Oxidative stress in bacteria and protein damage by reactive oxygen species / Int. Microbiol 3:3~8
Carneiro S / 2013 / Metabolic responses to recombinant bioprocesses in Escherichia coli / J. Biotechnol 164:396~408
Chatzifragkou A / 1718 / Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718 / Biotechnol. Bioeng 107:76~84
De La Cruz Rodriguez LC / 1990 / Damage of Escherichia coli cells by t-butylhydroperoxide involves the respiratory chain but is independent of the presence of oxygen / Biochim. Biophys. Acta 1015:510~516
De Mey M / 2007 / Minimizing acetate formation in E. coil fermentations / J. Ind. Microbiol. Biotechnol 34:689~700
Carneiro S / 2013 / Metabolic responses to recombinant bioprocesses in Escherichia coli / J. Biotechnol 164:396~408
Chatzifragkou A / 1718 / Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718 / Biotechnol. Bioeng 107:76~84
De La Cruz Rodriguez LC / 1990 / Damage of Escherichia coli cells by t-butylhydroperoxide involves the respiratory chain but is independent of the presence of oxygen / Biochim. Biophys. Acta 1015:510~516
De Mey M / 2007 / Minimizing acetate formation in E. coil fermentations / J. Ind. Microbiol. Biotechnol 34:689~700
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