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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호
- 발행연도
- 2016.12
- 수록면
- 2,179 - 2,183 (5page)
- DOI
- 10.4014/jmb.1606.06035
이용수
초록· 키워드
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Nitrilases pose attractive alternatives to the chemical hydrolysis of nitrile compounds. The activity of bacterial nitrilases towards substrate is intimately tied to the formation of large spiral-shaped oligomers. In the nitrilase CynD (cyanide dihydratase) from Bacillus pumilus, mutations in a predicted oligomeric surface region altered its oligomerization and reduced its activity. One mutant, CynD Y70C, retained uniform oligomer formation however it was inactive, unlike all other inactive mutants throughout that region all of which significantly perturbed oligomer formation. It was hypothesized that Y70 is playing an additional role necessary for CynD activity beyond influencing oligomerization. Here, we performed saturation mutagenesis at residue 70 and demonstrated that only tyrosine or phenylalanine is permissible for CynD activity. Furthermore, we show that other residues at this position are not only inactive, but have altered or disrupted oligomer conformations. These results suggest that Y70’s essential role in activity is independent of its role in the formation of the spiral oligomer.
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참고문헌 (17)
참고문헌 신청
Abou Nader M / 2012 / Directed evolution of cyanide degrading enzymes
Fisher FB / 1952 / Colorimetric determination of cyanide in stack gas and waste water / Anal. Chem 24:1440~1444
Jandhyala D / 2003 / CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies / Appl. Environ. Microbiol 69:4794~4805
Kimani SW / 2007 / Structure of an aliph atic amidase from Geobacillus pallidus RAPc8. Acta Crystallogr / D Biol. Crystallogr 63:1048~1058
Kumaran D / 2003 / Crystal structure of a putative CN hydrolase from yeast / Proteins 52:283~291
Fisher FB / 1952 / Colorimetric determination of cyanide in stack gas and waste water / Anal. Chem 24:1440~1444
Jandhyala D / 2003 / CynD, the cyanide dihydratase from Bacillus pumilus: gene cloning and structural studies / Appl. Environ. Microbiol 69:4794~4805
Kimani SW / 2007 / Structure of an aliph atic amidase from Geobacillus pallidus RAPc8. Acta Crystallogr / D Biol. Crystallogr 63:1048~1058
Kumaran D / 2003 / Crystal structure of a putative CN hydrolase from yeast / Proteins 52:283~291
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