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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Perspectives 제6권 제1호
- 발행연도
- 2015.2
- 수록면
- 9 - 13 (5page)
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초록· 키워드
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Objectives: Development of an efficacious vaccine against brucellosis has been a challenge for scientists for many years. At present, there is no licensed vaccine against human brucellosis. To overcome this problem, currently, antigenic determinants of Brucella cell wall such as Lipopolysaccharide (LPS) are considered as potential candidates to develop subunit vaccines. Methods: In this study, Brucella abortus LPS was used for conjugation to Neisseria meningitidis serogroup B outer membrane vesicle (OMV) as carrier protein using carbodiimide and adipic acid-mediated coupling and linking, respectively. Groups of eight BALB/c mice were injected subcutaneously with 10 ㎍LPS alone, combined LPS + OMV and conjugated LPS-OMV on 0 days, 14 days, 28 days and 42 days. Anti-LPS IgG was measured in serum. Results: The yield of LPS to OMV in LPS-OMV conjugate was 46.55%, on the basis of carbohydrate content. The ratio for LPS to OMV was 4.07. The LPS-OMV conjugate was the most immunogenic compound that stimulated following the first injection with increased IgG titer of ~ 5-fold and ~1.3-fold higher than that produced against LPS and LPS in noncovalent complex to OMV (LPS + OMV), respectively. The highest anti-LPS IgG titer was detected 2 weeks after the third injection (Day 42) of LPS-OMV conjugate. The conjugated compound elicited higher titers of IgG than LPS + OMV, that showed a 100-120-fold rise of anti-LPS IgG in mice. Conclusion: These results indicate that our conjugated LPS-OMV can be used as a brucellosis vaccine, but further investigation is required.
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참고문헌 (22)
참고문헌 신청
Sharifat Salmani A / 2009 / Serological evaluation of Brucella abortus S99 lipopolysaccharide extracted by an optimized method / Am J Infect Dis 5:11~16
Sharifat Salmani A / 2009 / Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant to induce specific antibody response against the lipopolysaccharide of Brucella abortus S99 / Ann Microbiol 59:145~149
Shapuri R / 2008 / Optimization of Brucella abortus fermenter cultural conditions and LPS extraction methods for antigen production / Res J Microbiol 3:1~8
Sh Yu / 2005 / Synthesis and characterization of lipooligosaccharide-based conjugate vaccines for serotype B Moraxella catarrhalis / Infect Immun 73:2790~2796
Sh Yu / 2007 / Biological and immunological characteristics of lipooligosaccharide-based conjugate vaccines for serotype C Moraxella catarrhalis / Infect Immun 75:2974~2980
Sharifat Salmani A / 2009 / Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant to induce specific antibody response against the lipopolysaccharide of Brucella abortus S99 / Ann Microbiol 59:145~149
Shapuri R / 2008 / Optimization of Brucella abortus fermenter cultural conditions and LPS extraction methods for antigen production / Res J Microbiol 3:1~8
Sh Yu / 2005 / Synthesis and characterization of lipooligosaccharide-based conjugate vaccines for serotype B Moraxella catarrhalis / Infect Immun 73:2790~2796
Sh Yu / 2007 / Biological and immunological characteristics of lipooligosaccharide-based conjugate vaccines for serotype C Moraxella catarrhalis / Infect Immun 75:2974~2980
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