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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 363 - 370 (8page)
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Using the Chinese cabbage (Brassica campestris) cultivar ‘Chun-goang’ as a host and turnip mosaic virus (TuMV) as a pathogen, we studied the effects of ambient temperature (13oC, 18oC, 23oC, 28oC and 33oC) on disease intensity and the speed of systemic infection. The optimal temperature for symptom expression of TuMV was 18-28oC. However, symptoms of viral infection were initiated at 23-28oC and 6 days post infection (dpi). Plants maintained at 33oC were systemically infected as early as 6 dpi and remained symptomless until 12 or 22 dpi, depending on growth stage at the time of inoculation. It took 45 days for infection of plants grown at 13oC. Quantitative real-time polymerase chain reaction (q-PCR) results showed that the accumulation of virus coat protein was greater in plants grown at 23-28oC. The speed of systemic infection increased linearly with rising ambient temperature, up to 23oC. The zero-infection temperature was 10.1oC. To study the effects of abruptly elevated temperatures on systemic infection, plants inoculated with TuMV were maintained at 10oC for 20 d; transferred to a growth chamber at temperatures of 13oC, 18oC, 23oC, 28oC, or 33oC for 1, 2, or 3 d; and then moved back to 10oC. The numbers of plants infected increased as duration of exposure to higher temperatures and dpi increased.
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