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논문 기본 정보

자료유형
학술저널
저자정보
Noormohammadi Nafiseh (Department of Plant Production and Genetic Engineering Faculty of Agriculture Lorestan University) Ismaili Ahmad (Department of Plant Production and Genetic Engineering Faculty of Agriculture Lorestan University) Sobhani Najafabadi Ahmad (Agricultural Biotechnology Research Institute of Iran-Isfahan Branch Agricultural Research Education and Extension Organization) Nazarian-Firouzabadi Farhad (Department of Plant Production and Genetic Engineering Faculty of Agriculture Lorestan University)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제2호
발행연도
2023.4
수록면
229 - 241 (13page)
DOI
10.1007/s11816-022-00794-6

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Hypericin and hyperforin are the most important secondary metabolites of Hypericum perforatum, which are fundamen- tally effective and useful in treating depression. However, the biosynthetic pathways of such important compounds have not been well recognized. In this research, RNA-seq data of H. perforatum were obtained from the European nucleotide archive (ENA) of EMBL-EBI and then WGCNA (weighted correlation network analysis) package in R was utilized to analyze the hypericin and hyperforin biosynthesis gene networks to identify the most effective genes in the biosynthetic pathway of these metabolites. Furthermore, the foliar application of different methyl jasmonate concentrations including zero (control), 200 μM and 500 μM was performed on H. perforatum. Then, the content of total phenol, flavonoids, hypericin and hyperforin were measured at 12, 24, 48 and 72 h, 7 and 14 days after treatment. The results showed that the highest content of total phenol and flavonoid were observed at the concentration of 500 μM of MeJA at 14 days after spraying, while the highest content of hypericin and hyperforin was accumulated at 200 μM of MeJA after treatment for 14 days. Moreover, qRT-PCR experiment analysis showed that the expression of several transcripts including polyketides synthase 2 (DN112064_c2_g1_i2), dof zinc finger (DN123509_c0_g1_i2), MYB-related 308 (DN118134_c3_g1_i2), geranylgeranyl pyrophosphate (DN125559_c3_ g3_i5) and polyketides synthase 1 (DN126440_c0_g1_i2) were upregulated after spraying 200 μM of MeJA.

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