지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 환경독성보건학회 Environmental Analysis Health and Toxicology Environmental Analysis Health and Toxicology Vol.39 No.4
- 발행연도
- 2024.12
- 수록면
- 39 - 53 (15page)
이용수
초록· 키워드
오류제보하기
Snakebite by a cobra is considered neurotoxic as the cause of neuromuscular paralysis mediated by low molecular weight toxins, which are major toxin components of cobra. However, these toxins represent a problem in generating antibodies owing to their low immunogenicity. Developing complementary strategies to improve the antibody response could be a useful approach to creating better therapeutic antivenoms with higher neutralizing potencies. To develop simple immunization strategies for more potent antivenoms by studying the effects of combining crude cobra venom and toxin fraction in a complementary way. The evaluation of specific cell immunology and cytokine mediators for relevant immune responses will be measured in a rabbit model using four simple immunization strategies. Flow cytometry will be used to quantify the number of B and T cells, and qRT-PCR will be used to ascertain the cytokine genes expressed. B cells with anti-CD20 were seen on D14, and a booster dose was insufficient to maximize the antibodies. Conversely, anti-CD5 for T cells decreased periodically but remained stable. Using a mixture of crude cobra venom and its <10 kDa fraction, peak expression of pro-inflammatory cytokine genes was seen in D42 or D58, with a rise of 4 and 6 folds. Similarly, gene expression of pro-inflammatory cytokines was greater than that of anti-inflammatory cytokines (IL-4 and IL-10), which were up-regulated after D42. Thus, immunization with both the crude and its <10 kDa fraction of cobra venom seems to have synergistic effects that boost cytokines, activate the immune system, and cause lymphocyte differentiation.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Conclusions
References
참고문헌 (31)
참고문헌 신청
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Utkin YN. Animal venom studies: current benefits and future developments. World J Biol Chem 2015;6(2):28-33. https://doi.org/10.4331/wjbc.v6.i2.28
Ratanabanangkoon K, Tan KY, Eursakun S, Tan CH, Simsiriwong P, Pamornsakda T, et al. A simple and novel strategy for the production of a pan-specific antiserum against elapid snakes of Asia. PLoS Negl Trop Dis 2016;10(4):e0004565. https://doi.org/10.1371/journal.pntd.0004565
Castro KL, Duarte CG, Ramos HR, Machado de Avila RA, Schneider FS, Oliveira D, et al. Identification and characterization of B-cell epitopes of 3FTx and PLA(2) toxins from Micrurus corallinus snake venom. Toxicon 2015;93:51-60. https://doi.org/10.1016/j.toxicon.2014.10.015
Liu BS, Jiang BR, Hu KC, Liu CH, Hsieh WC, Lin MH, et al. Development of a broad-spectrum antiserum against cobra venoms using recombinant three-finger toxins. Toxins (Basel) 2021;13(8):556. https://doi.org/10.3390/toxins13080556
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