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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제7호
- 발행연도
- 2008.1
- 수록면
- 1,335 - 1,341 (7page)
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The cell wall material from fruiting bodies of Laetiporus
sulphureus has been suggested as a new alternative to
mutan for the mutanase induction in Trichoderma harzianum.
Structural analyses revealed that the cell wall fraction
from this polypore fungus contained 56.3% of (1→3)-
linked α-glucans. When the strain T. harzianum F-340 was
grown on a cell wall preparation from L. sulphureus, the
maximal enzyme productivity obtained after 3 days of
cultivation was 0.71 U/ml. This yield was about 1.8-fold
higher than that achieved on mutan, known so far as
the best, but expensive and inaccessible, inducer of
mutanase production. Cell-wall-induced mutanase showed
a high hydrolytic potential in reaction with a dextranasepretreated
mutan, where maximal degrees of saccharification
and solubilization of this biopolymer (80% and 100%,
respectively) were reached in 3 h at 45oC. The mutanase
preparation was also effective in degradation of streptococcal
mutan and its removal from oral biofilms, especially in a
mixture with dextranase.
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참고문헌 (22)
참고문헌 신청
Davoli, P. / 2005 / Laetiporic acids, a family of non-carotenoid polyene pigments from fruit bodies and liquid cultures of Laetiporus sulphureus / Phytochemistry 66 : 817 ~ 8
Ershova, E. Y. / 2003 / Antimicrobial activity of Laetiporus sulphureus strains in submerged culture. Antibiot / Khimioter 48 : 18 ~ 22
Grun, C. H. / 2003 / Evidence for two conserved mechanisms of cell-wall α-glucan biosynthesis in fungi / University of Utrecht : 116 ~ 132
Guggenheim, B. / 1972 / Purification and properties of an α-(1-3) glucanohydrolase from Trichoderma harzianum. J. Dent / Res 51 : 394 ~ 402
Inoue, M. / 1990 / Inhibition of dental plaque formation by mouthwash containing an endo-α-1,3 glucanase. Clin. Prevent / Dent 12 : 10 ~ 14
Ershova, E. Y. / 2003 / Antimicrobial activity of Laetiporus sulphureus strains in submerged culture. Antibiot / Khimioter 48 : 18 ~ 22
Grun, C. H. / 2003 / Evidence for two conserved mechanisms of cell-wall α-glucan biosynthesis in fungi / University of Utrecht : 116 ~ 132
Guggenheim, B. / 1972 / Purification and properties of an α-(1-3) glucanohydrolase from Trichoderma harzianum. J. Dent / Res 51 : 394 ~ 402
Inoue, M. / 1990 / Inhibition of dental plaque formation by mouthwash containing an endo-α-1,3 glucanase. Clin. Prevent / Dent 12 : 10 ~ 14
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