지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제8호
- 발행연도
- 2009.1
- 수록면
- 818 - 822 (5page)
이용수
초록· 키워드
오류제보하기
A β-1,3-1,4-glucanase from the fungus Laetiporus sulphureus
var. miniatus was purified as a single 26 kDa band by
ammonium sulfate precipitation, HiTrap Q HP, and UNO
Q ion-exchange chromatography, with a specific activity of
29U/mg. The molecular mass of the native enzyme was 52 kDa
as a dimer by gel filtration. β-1,3-1,4-Glucanase showed
optimum activity at pH 4.0 and 75oC. The half-lives of the
enzyme at 70oC and 75oC were 152 h and 22 h, respectively.
The enzyme showed the highest activity for barley β-
glucan as β-1,3-1,4-glucan among the tested polysaccharides
and p-nitrophenyl-β-D-glycosides with a Km of 0.67 mg/ml,
a kcat of 13.5 s-1 and a kcat/Km of 20 mg/ml/s.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
[학술지(정기간행물
Bielecki, S. / 1991 / Microbial β-glucanases different from cellulases / Crit. Rev. Biotechnol 100 : 275 ~ 304
Celestino, K. R. / 2006 / Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry / BM
Erfle, J. D. / 1988 / Purification and properties of a 1,3-1,4-β-D-glucanase (lichenase, 1,3-1,4-β-D-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes c
Fincher, G. B. / 1975 / Morphology and chemical composition of barley endosperm cell walls / J. Inst. Brew 81 : 116 ~ 122
Bielecki, S. / 1991 / Microbial β-glucanases different from cellulases / Crit. Rev. Biotechnol 100 : 275 ~ 304
Celestino, K. R. / 2006 / Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry / BM
Erfle, J. D. / 1988 / Purification and properties of a 1,3-1,4-β-D-glucanase (lichenase, 1,3-1,4-β-D-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes c
Fincher, G. B. / 1975 / Morphology and chemical composition of barley endosperm cell walls / J. Inst. Brew 81 : 116 ~ 122
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