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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Journal of Food Science and Nutrition Journal of Food Science and Nutrition Vol.4 No.1
- 발행연도
- 1999.3
- 수록면
- 79 - 83 (5page)
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초록· 키워드
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A potentiometric biosensor employing a CO₃^(2-) ion-selective electrode (ISE) and malic enzyme immobilization in a flow injection analysis (FIA) system was constructed. Analytical parameters were optimized for L-malate determination. The CO₃^(2-)-ISE-FIA system was composed of a pump, an injector, a malic enzyme (EC 1.1.1.40) reactor, a CO₃^(2-) ion-selective electrode, a pH/㎷ meter and a recorder. Cofactor NADP was also injected with substrate for the enzyme reaction into the system. Optimized analytical parameters for L-malate determination in the CO₃^(2-)-ISE-FIA system were as follows: flow rate, 14.5 ml/hr; sample injection volume, 100 μl; enzyme loading in the reactor, 20 units; length of the enzyme reactor, 7 ㎝; tubing length from the enzyme reactor to the detector as a geometric factor in FIA, 15 ㎝. The response time for measuring the entire L-malate concentration range 10^(-2)~10^(-5) mol/L; 4 injections) was <15 minutes. In this CO₃^(2-)-ISE-FIA system, the potential differences due to the formation of CO₃^(2-) by the reaction of malic enzyme on L-malate were correlated to L-malate concentration in the range of 10^(-2)~10^(-5) mol/L; the detection limit was 10^(-5) mol/L. This potentiometric CO₃^(2-)-ISE-FIA system was found to be useful for L-malate measurement.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2009-511-018075008