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Mexican lime (Citrus aurantifolia L.) is the most sensitive citrus plant to freezing. To study the effect of water stress on development of freezing tolerance of lime seedlings, changes of chlorophyll content, total soluble sugar, electrolyte leakage (EL), proline, and chlorophyll fluorescence parameters, as indicators of freezing damage were evaluated. The seedlings at the stage of 7-9 leaves were exposed to four levels of field capacity (FC) including 100%, 75%, 50%, and 25% FC. Then, water stressed plants were subjected to -2, -4, -6, and -8℃ freezing temperatures for 12 hours. The results showed an increase in EL and total soluble sugar during freezing stress, whereas chlorophyll content, efficiency of photosystem II (Fv/Fm), and electron transfer rate (ETR) considerably decreased. Within the water stressed treatments, 25% FC decreased EL to 71.5% of control, while chlorophyll content, soluble sugars, Fv/Fm, and ETR were significantly increased. Therefore, as a vital indicator, 25% FC water stress could improve freezing tolerance of lime seedlings.

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