지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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Kv 4.2 ion channel protein has an ability to open at subthreshold membrane potentials and to recover quickly from inactivation. That is very important for neuronal signal transmission in vertebrate brain. In order to purify Kv 4.2 protein, the novel purification methods were experimented. The purification procedure utilized chromatography on DE-52 ion exchange column and affinity chromatography on a WGA-Sepharose 4B, and Kv 4.2 affinity column chromatography. It was found that 0.5% (wt./vol.) Triton X-100 detergent in lysis buffer worked well for Kv 4.2 protein solubilization from rat brain membrane. Protein quantitative determination was conducted by BCA method at 562 nm for each purification step to avoid determination interference of protein at 280 nm by detergent. The confirmation of Kv 4.2 existence and amount is performed using by SDS-PAGE/immunoblotting or 96-well dot blotting. The Kv 4.2 without interacting protein that contains carbohydrate, was purified from novel biochemical 3-steps purification method for further research.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (45)
참고문헌 신청
Alonso G / 1997 / Clustering of KV4.2 potassium channels in postsynaptic membrane of rat supraoptic neurons: an ultrastructural study / Neuroscience 77:617~621
Baldwin TJ / 1991 / Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channels / Neuron 7:471~483
Barry DM / 1995 / Differential expression of voltage-gated K+ channel subunits in adult rat heart. Relation to functional K+ channels / Circulation Research 77:361~369
Bekele-Arcuri Z / 1996 / Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides / Neuropharmacology 35:851~865
Castle NA / 1989 / Toxins in the characterization of potassium channels / Trends in Neuroscience 12:59~65
Baldwin TJ / 1991 / Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channels / Neuron 7:471~483
Barry DM / 1995 / Differential expression of voltage-gated K+ channel subunits in adult rat heart. Relation to functional K+ channels / Circulation Research 77:361~369
Bekele-Arcuri Z / 1996 / Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides / Neuropharmacology 35:851~865
Castle NA / 1989 / Toxins in the characterization of potassium channels / Trends in Neuroscience 12:59~65
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UCI(KEPA) : I410-ECN-0101-2013-510-002648580