The changes in quality and physiological activity of royal jelly (RJ) stored at room temperatures, 4°C, and -20°C under dark and light conditions for up to 6 months were investigated. The results showed that 10-HDA, total amino acid, free amino acid of RJ changed significantly during storage at room temperature both dark and light and 4°C, but not at -20°C. The physiological activity of RJ such as antioxidative activity using ABTS radical scavenging activity and nitric oxide scavenging activity at 50 μg/mL were decreased significantly during storage at room temperature and 4°C after 3 months, but not at -20°C. The human dermal fibroblasts (HDF) proliferations was decreased significantly durings torage at room temperature after 3 months, but not 4°C and -20°C. The results indicated that the quality deterioration and physiological activity of RJ during storage was due to the Maillard browning reaction.