지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.17 No.4
- 발행연도
- 2012.12
- 수록면
- 274 - 279 (6page)
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초록· 키워드
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For the quantitative analysis of genetically modified (GM) maize in processed foods, primer sets and probes based on the 35S promoter (p35S), nopaline synthase terminator (tNOS), p35S-hsp70 intron, and zSSIIb gene encoding starch synthase II for intrinsic control were designed. Polymerase chain reaction (PCR) products (80～101bp) were specifically amplified and the primer sets targeting the smaller regions (80 or 81 bp) were more sensitive than those targeting the larger regions (94 or 101 bp). Particularly, the primer set 35F1-R1 for p35S targeting 81 bp of sequence was even more sensitive than that targeting 101 bp of sequence by a 3-log scale. The target DNA fragments were also specifically amplified from all GM labeled food samples except for one item we tested when 35F1-R1 primer set was applied. A reference plasmid pGMmaize (3 kb) including the smaller PCR products for p35S, tNOS, p35S-hsp70 intron, and the zSSIIb gene was constructed for real-time PCR (RT-PCR). The linearity of standard curves was confirmed by using diluents ranging from 2×10<SUP>1</SUP>～10<SUP>5</SUP> copies of pGMmaize and the R<SUP>2</SUP> values ranged from 0.999～1.000. In the RT-PCR, the detection limit using the novel primer/probe sets was 5pg of genomic DNA from MON810 line indicating that the primer sets targeting the smaller regions (80 or 81 bp) could be used for highly sensitive detection of foreign DNA fragments from GM maize in processed foods.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - LEE, SEONG-HUN / 2006 / Quantitative Analysis of Two Genetically Modified Maize Lines by Real-Time PCR / Journal of Microbiology and Biotechnology 16 (2) : 205 ~ 211
2. [학술지(정기간행물)] - James C. / 2011 / Global status of commercialized biotech/ GM crops / ISAAA Brief 43
3. [학술지(정기간행물)] - Ahmed FE / 2002 / Detection of genetically modified organisms in foods / Trends Biotechnol 20 : 215 ~ 223
4. [학술지(정기간행물)] - Fantozzi A / 2007 / First application of a microsphere-based immunoassay to the detection of genetically modified organisms (GMOs): quantification of Cry1Ab protein in genetically modified maize / J Agric Food Chem 55 : 1071 ~ 1076
5. [학술지(정기간행물)] - Hernández M / 2005 / Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21 / J Agric Food Chem 53 : 3333 ~ 3337
2. [학술지(정기간행물)] - James C. / 2011 / Global status of commercialized biotech/ GM crops / ISAAA Brief 43
3. [학술지(정기간행물)] - Ahmed FE / 2002 / Detection of genetically modified organisms in foods / Trends Biotechnol 20 : 215 ~ 223
4. [학술지(정기간행물)] - Fantozzi A / 2007 / First application of a microsphere-based immunoassay to the detection of genetically modified organisms (GMOs): quantification of Cry1Ab protein in genetically modified maize / J Agric Food Chem 55 : 1071 ~ 1076
5. [학술지(정기간행물)] - Hernández M / 2005 / Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21 / J Agric Food Chem 53 : 3333 ~ 3337
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UCI(KEPA) : I410-ECN-0101-2014-511-000596568