Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

Francine C. Paim; Aleksandro S. Da Silva; Carlos Breno V. Paim; Raqueli T. França; Márcio M. Costa; Marta M. M. F. Duarte; Manuela B. Sangoi; Rafael N. Moresco; Silvia G. Monteiro; Sonia Terezinha A. Lopes

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저자정보: Francine C. Paim (Universidade Federal de Santa Maria) Aleksandro S. Da Silva (Universidade do Estado de Santa Catarina) Carlos Breno V. Paim (Universidade Federal de Santa Maria) Raqueli T. França (Universidade Federal de Santa Maria) Márcio M. Costa (Universidade Federal de Santa Maria) Marta M. M. F. Duarte (Lutheran University of Brazil-ULBRA) Manuela B. Sangoi (Universidade Federal de Santa Maria) Rafael N. Moresco (Universidade Federal de Santa Maria) Silvia G. Monteiro (Universidade Federal de Santa Maria) Sonia Terezinha A. Lopes (Universidade Federal de Santa Maria)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.51 No.1

발행연도: 2013.2

수록면: 133 - 137 (5page)

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This study aimed to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NOx) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-γ, TNF-α, IL-1, IL-6, and NOx. Cytokines were assessed by ELISA quantitative sandwich technique, and NOx was measured by the modified Griess method. Cytokine levels (IFN-γ, TNF-α, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NOx were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

#Rangelia vitalii #IFN-γ #TNF-α #IL-1 #IL-6 #nitric oxide #immunity #rangeliosis

참고문헌 (38)

참고문헌 신청

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