지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.12
- 수록면
- 57 - 64 (8page)
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초록· 키워드
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Purpose: This study was conducted to determine cell viability and differentiation capability of human periodontal ligament (PDL) cells and to elucidate the effects of cryopreservation on the activity of human third molar PDL cells by comparing PDL cells with and without cryopreservation.
Materials and Methods: Human PDL fibroblasts obtained from immature third molars were cultured and divided into two groups. The experimental group was cryopreserved with a slow freezing rate of 0.5℃/min from 4℃ to –35℃ followed by plunging in liquid nitrogen at –196℃ and cultured after fast thawing. The control group was cultured without cryopreservation. Cell viability, growth capacity and morphology were evaluated in both groups. Bivariate statistics were used to compare 2 groups and linear mixed model analysis was used to investigate the growth trends difference over time.
Result: Cell viability and growth capacity were not significantly different between the 2 groups (P>0.05). Cultured cell of both groups showed fibroblast-like in appearance, and there were no significant differences in morphology between 2 groups. The mixed model analysis revealed no significant difference of growth capacity between 2 groups over time (β=–0.0009; P=0.138).
Conclusion: This study demonstrates that cryopreservation under control does not affect the biological properties of PDL cells, supporting the feasibility of autotransplantation of cryopreserved impacted third molars.
Materials and Methods: Human PDL fibroblasts obtained from immature third molars were cultured and divided into two groups. The experimental group was cryopreserved with a slow freezing rate of 0.5℃/min from 4℃ to –35℃ followed by plunging in liquid nitrogen at –196℃ and cultured after fast thawing. The control group was cultured without cryopreservation. Cell viability, growth capacity and morphology were evaluated in both groups. Bivariate statistics were used to compare 2 groups and linear mixed model analysis was used to investigate the growth trends difference over time.
Result: Cell viability and growth capacity were not significantly different between the 2 groups (P>0.05). Cultured cell of both groups showed fibroblast-like in appearance, and there were no significant differences in morphology between 2 groups. The mixed model analysis revealed no significant difference of growth capacity between 2 groups over time (β=–0.0009; P=0.138).
Conclusion: This study demonstrates that cryopreservation under control does not affect the biological properties of PDL cells, supporting the feasibility of autotransplantation of cryopreserved impacted third molars.
목차
Introduction
Materials and Methods
Result
Discussion
Conclusion
References
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Andreasen JO / 1990 / A long-term study of 370 autotransplanted premolars. Part II. Tooth survival and pulp healing subsequent to transplantation / Eur J Orthod 12 : 14 ~ 24
[학술지(정기간행물)] - Czochrowska EM / 2002 / Outcome of tooth transplantation: survival and success rates 17-41 years posttreatment / Am J Orthod Dentofacial Orthop 121 : 110 ~ 119
[학술지(정기간행물)] - Oh YH / 2005 / Cryopreservation of human teeth for future organization of a tooth bank: a preliminary study / Cryobiology 51 : 322 ~ 329
[학술지(정기간행물)] - Schwartz O / 1985 / Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing / Int J Oral Surg 14 : 350 ~ 361
[학술지(정기간행물)] - Temmerman L / 2008 / Influence of cryopreservation on human periodontal ligament cells in vitro / Cell Tissue Bank 9 : 11 ~ 18
[학술지(정기간행물)] - Czochrowska EM / 2002 / Outcome of tooth transplantation: survival and success rates 17-41 years posttreatment / Am J Orthod Dentofacial Orthop 121 : 110 ~ 119
[학술지(정기간행물)] - Oh YH / 2005 / Cryopreservation of human teeth for future organization of a tooth bank: a preliminary study / Cryobiology 51 : 322 ~ 329
[학술지(정기간행물)] - Schwartz O / 1985 / Cryopreservation before replantation of mature teeth in monkeys. (II). Effect of preincubation, different freezing and equilibration rates and endodontic treatment upon periodontal healing / Int J Oral Surg 14 : 350 ~ 361
[학술지(정기간행물)] - Temmerman L / 2008 / Influence of cryopreservation on human periodontal ligament cells in vitro / Cell Tissue Bank 9 : 11 ~ 18
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UCI(KEPA) : I410-ECN-0101-2016-515-002234281