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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.43 No.1
- 발행연도
- 2016.3
- 수록면
- 109 - 115 (7page)
이용수
초록· 키워드
오류제보하기
Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 ㎎/㎏ body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 ㎎/㎏ body weight in both sexes.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (15)
참고문헌 신청
Arun KHS, Kaul CL, Ramarao P. 2005. Green fluorescent proteins in receptor research: an emerging tool for drugdiscovery. Journal of Pharmacological and Toxicological Methods 51(1):1-23.
Cinelli RA, Ferrari A, Pellegrini V, Tyagi M, Giacca M, Beltram F. 2000. The enhanced green fluorescent protein as a tool for theanalysis of protein dynamics and localization: local fluorescence study at the single-molecule level. Photochemistry andPhotobiology 71(6):771.
Imamura M, Nakai J, Inoue S, Quan GX, Kanda T, Tamura T. 2003. Targeted gene expression using the GAL4/UAS system in thesilkworm Bombyx mori. Genetics 165(3):1329-1340.
Joo KI, Wang P. 2008. Visualization of targeted transduction by engineered lentiviral vectors. Gene Therapy 15(20):1384-1396.
Kim SW, Kang MU, Kang SW, Yun EY, Choi KH, Kim SR, Park SW, Nho SK, Goo TW. 2013a. Modification of the commercialsilkworm eggs adequate for silkworm transgenesis. Journal of Sericultural and Entomological Science 51(1):73-77.
Cinelli RA, Ferrari A, Pellegrini V, Tyagi M, Giacca M, Beltram F. 2000. The enhanced green fluorescent protein as a tool for theanalysis of protein dynamics and localization: local fluorescence study at the single-molecule level. Photochemistry andPhotobiology 71(6):771.
Imamura M, Nakai J, Inoue S, Quan GX, Kanda T, Tamura T. 2003. Targeted gene expression using the GAL4/UAS system in thesilkworm Bombyx mori. Genetics 165(3):1329-1340.
Joo KI, Wang P. 2008. Visualization of targeted transduction by engineered lentiviral vectors. Gene Therapy 15(20):1384-1396.
Kim SW, Kang MU, Kang SW, Yun EY, Choi KH, Kim SR, Park SW, Nho SK, Goo TW. 2013a. Modification of the commercialsilkworm eggs adequate for silkworm transgenesis. Journal of Sericultural and Entomological Science 51(1):73-77.
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UCI(KEPA) : I410-ECN-0101-2016-480-002738522