지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Among the several agents causing carbapenem resistance of Acinetobacter baumannii, the most common cause is OXA-23 β-lactamase, which is known to hydrolyze carbapenem. To effectively control dissemination of carbapenemresistant Acinetobacter baumannii (CRAB), development of both rapid and easy-to-use detection methods are required. The aim of this study is to develop a novel immunochromatographic assay (ICA) for rapid detection of OXA-23 β-lactamase. Of the seven monoclonal antibodies (mAbs) screened by ELISA, four mAbs (4G6, 4H6, 6G4, 9A4) exhibited high reactivity. Of these four specific antibodies, the combination of 6G4/4G6 showed the greatest reactivity and this combination of mAbs (6G4/4G6 mAbs) was used to develop the OXA-23 β-lactamase ICA. Of 102 A. baumannii isolates tested, the OXA-23 β-lactamase ICA results were consistent with PCR analysis except one false positive and one false negative isolate. The overall sensitivity and specificity were 98.36% and 97.56%, respectively. In conclusion, to the best of our knowledge, we have developed the first specific antibody set to detect OXA-23 β-lactamase using an ICA kit. This novel ICA can be used as a reliable and easy-to-use immunological assay for detection of OXA-23 β-lactamase producing CRAB in clinical laboratories.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] - Bergmans HEN / 1981 / Transformation in Escherichia coli: stages in the process / J Bacteriol 146 : 564 ~ 570
[기타자료] - Clinical and Laboratory Standard Institute / 2012 / Performance for antimicrobial susceptibility testing; twenty two informational supplement (M100-S22) Wayne / CLSI
[학술지(정기간행물)] - Hochuli E / 1988 / Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent / Nature Biotechnology 6 : 1321 ~ 1325
[학술지(정기간행물)] - 정석훈 / 2006 / Outbreaks of Imipenem-Resistant Acinetobacter baumannii Producing Carbapenemases in Korea / The Journal of Microbiology 44 (4) : 423 ~ 431
[학술지(정기간행물)] - 지길용 / 2014 / Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci / Journal of Microbiology and Biotechnology 24 (3) : 427 ~ 430
[기타자료] - Clinical and Laboratory Standard Institute / 2012 / Performance for antimicrobial susceptibility testing; twenty two informational supplement (M100-S22) Wayne / CLSI
[학술지(정기간행물)] - Hochuli E / 1988 / Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent / Nature Biotechnology 6 : 1321 ~ 1325
[학술지(정기간행물)] - 정석훈 / 2006 / Outbreaks of Imipenem-Resistant Acinetobacter baumannii Producing Carbapenemases in Korea / The Journal of Microbiology 44 (4) : 423 ~ 431
[학술지(정기간행물)] - 지길용 / 2014 / Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci / Journal of Microbiology and Biotechnology 24 (3) : 427 ~ 430
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
OXA-48 계열 Carbapenemase 생성 장내세균의 출현과 확산
의료관련감염관리
2015 .01
-
Four genotypes of carbapenem-resistant Acinetobacter baumannii strains lacking OXA-23 production in Korea
Annals of Clinical Microbiology
2024 .06
-
In Vitro Synergistic Effects of Antimicrobial Combinations on Extensively Drug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Isolates
Annals of Laboratory Medicine
2016 .01
-
Prevalence, Antimicrobial Susceptibility, and Carbapenem Resistance of Non-baumannii Acinetobacter Species in Three Korean Hospitals
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2024 .06
-
Distribution of β-Lactamase Genes Among Carbapenem-Resistant Klebsiella pneumoniae Strains Isolated From Patients in Turkey
Annals of Laboratory Medicine
2015 .01
-
Profiling of antimicrobial resistance and plasmid replicon types in β-lactamase producing Escherichia coli isolated from Korean beef cattle
Journal of Veterinary Science
2015 .12
-
NDM-5 and OXA-48 Co-producing Uropathogenic Escherichia coli Isolate: First Case in Korea
Annals of Laboratory Medicine
2018 .01
-
Interleukin-10 is critical for the protection of mice from lethality by lung infection of Acinetobacter baumannii
한국실험동물학회 학술발표대회 논문집
2015 .02
-
Seasonal and Temperature-Associated Increase in Community-Onset Acinetobacter baumannii Complex Colonization or Infection
Annals of Laboratory Medicine
2018 .01
-
Rapid Identification of OXA-48-like, KPC, NDM, and VIM Carbapenemase-Producing Enterobacteriaceae From Culture: Evaluation of the RESIST-4 O.K.N.V. Multiplex Lateral Flow Assay
Annals of Laboratory Medicine
2020 .01
-
Increasing Carbapenem-Resistant Gram-Negative Bacilli and Decreasing Metallo-β-Lactamase Producers over Eight Years from Korea
Yonsei Medical Journal
2015 .01
-
The Nod2 in essential for host immune defense against Acinetobacter baumannii infection
한국실험동물학회 학술발표대회 논문집
2016 .02
-
Prevalence and Molecular Characteristics of Carbapenemase-Producing Enterobacteriaceae From Five Hospitals in Korea
Annals of Laboratory Medicine
2016 .01
-
The First Klebsiella pneumoniae Isolate Co-Producing OXA-48 and NDM-1 in Turkey
Annals of Laboratory Medicine
2015 .01
-
A Case of Community-Acquired Pneumonia Caused by Multidrug-Resistant Acinetobacter baumannii in Korea
Infection and Chemotherapy
2017 .01
-
Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing Acinetobacter baumannii
Annals of Laboratory Medicine
2020 .01
-
Abrupt Increase in Rate of Imipenem Resistance in Acinetobacter baumannii Complex Strains Isolated from General Hospitals in Korea and Correlation With Carbapenem Administration During 2002–2013
Annals of Laboratory Medicine
2018 .01
-
Optimizing Treatment for Carbapenem-Resistant Acinetobacter baumannii Complex Infections: A Review of Current Evidence
Infection and Chemotherapy
2024 .06
-
카바페넴분해효소 생성 장내세균 검출을 위한 Multiplex PCR의 개발 및 평가
Annals of Clinical Microbiology
2019 .01
-
Colistin, Hot Potato for the Therapy of Carbapenem-resistant Acinetobacter baumannii infections
Journal of Korean Medical Science
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-510-000852066