지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 196 - 202 (7page)
이용수
초록· 키워드
오류제보하기
Background: Ginsenoside Rd (GSRd), a main component of the root of Panax ginseng, exhibits antiinflammation functions and decreases infarct size in many injuries and ischemia diseases such as focal cerebral ischemia. M1 Macrophages are regarded as one of the key inflammatory cells having functions for disease progression.
Methods: To investigate the effect of GSRd on renal ischemia/reperfusion injury (IRI) and macrophage functional status, and their regulatory role on mouse polarized macrophages in vitro, GSRd (10-100 mg/kg) and vehicle were applied to mice 30 min before renal IRI modeling. Renal functions were reflected by blood serum creatinine and blood urea nitrogen level and histopathological examination. M1 polarized macrophages infiltration was identified by flow cytometry analysis and immunofluorescence staining with CD11b<SUP>+</SUP>, iNOS<SUP>+</SUP>/interleukin-12/tumor necrosis factor-a labeling. For the in vitro study, GSRd (10-100 μg/mL) and vehicle were added in the culture medium of M1 macrophages to assess their regulatory function on polarization phenotype.
Results: In vivo data showed a protective role of GSRd at 50 mg/kg on Day 3. Serum level of serum creatinine and blood urea nitrogen significantly dropped compared with other groups. Reduced renal tissue damage and M1 macrophage infiltration showed on hematoxylineeosin staining and flow cytometry and immunofluorescence staining confirmed this improvement. With GSRd administration, in vitro cultured M1 macrophages secreted less inflammatory cytokines such as interleukin-12 and tumor necrosis factor-α. Furthermore, macrophage polarization-related pancake-like morphology gradually changed along with increasing concentration of GSRd in the medium.
Conclusion: These findings demonstrate that GSRd possess a protective function against renal ischemia/reperfusion injury via downregulating M1 macrophage polarization.
Methods: To investigate the effect of GSRd on renal ischemia/reperfusion injury (IRI) and macrophage functional status, and their regulatory role on mouse polarized macrophages in vitro, GSRd (10-100 mg/kg) and vehicle were applied to mice 30 min before renal IRI modeling. Renal functions were reflected by blood serum creatinine and blood urea nitrogen level and histopathological examination. M1 polarized macrophages infiltration was identified by flow cytometry analysis and immunofluorescence staining with CD11b<SUP>+</SUP>, iNOS<SUP>+</SUP>/interleukin-12/tumor necrosis factor-a labeling. For the in vitro study, GSRd (10-100 μg/mL) and vehicle were added in the culture medium of M1 macrophages to assess their regulatory function on polarization phenotype.
Results: In vivo data showed a protective role of GSRd at 50 mg/kg on Day 3. Serum level of serum creatinine and blood urea nitrogen significantly dropped compared with other groups. Reduced renal tissue damage and M1 macrophage infiltration showed on hematoxylineeosin staining and flow cytometry and immunofluorescence staining confirmed this improvement. With GSRd administration, in vitro cultured M1 macrophages secreted less inflammatory cytokines such as interleukin-12 and tumor necrosis factor-α. Furthermore, macrophage polarization-related pancake-like morphology gradually changed along with increasing concentration of GSRd in the medium.
Conclusion: These findings demonstrate that GSRd possess a protective function against renal ischemia/reperfusion injury via downregulating M1 macrophage polarization.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Kim YJ / 2013 / Efficient thermal deglycosylation of ginsenoside Rd and its contribution to the improved anticancer activity of ginseng / J Agric Food Chem 61 : 9185 ~ 9191
[학술지(정기간행물)] - Ye R / 2011 / Ginsenoside Rd attenuates early oxidative damage and sequential inflammatory response after transient focal ischemia in rats / Neurochem Int 58 : 391 ~ 398
[학술지(정기간행물)] - Wang Y / 2013 / Ginsenoside Rd attenuates myocardial ischemia/reperfusion injury via Akt/GSK-3b signaling and inhibition of the mitochondria-dependent apoptotic pathway / PLoS One 8 : e70956 ~
[학술지(정기간행물)] - Vanden Hoek TL / 2003 / Reperfusion, not simulated ischemia, initiates intrinsic apoptosis injury in chick cardiomyocytes / Am J Physiol Heart Circ Physiol 284 : H141 ~ H150
[학술지(정기간행물)] - Lee JA / 1992 / Changes in intracellular free calcium concentration during long exposures to simulated ischemia in isolated mammalian ventricular muscle / Circ Res 71 : 58 ~ 69
[학술지(정기간행물)] - Ye R / 2011 / Ginsenoside Rd attenuates early oxidative damage and sequential inflammatory response after transient focal ischemia in rats / Neurochem Int 58 : 391 ~ 398
[학술지(정기간행물)] - Wang Y / 2013 / Ginsenoside Rd attenuates myocardial ischemia/reperfusion injury via Akt/GSK-3b signaling and inhibition of the mitochondria-dependent apoptotic pathway / PLoS One 8 : e70956 ~
[학술지(정기간행물)] - Vanden Hoek TL / 2003 / Reperfusion, not simulated ischemia, initiates intrinsic apoptosis injury in chick cardiomyocytes / Am J Physiol Heart Circ Physiol 284 : H141 ~ H150
[학술지(정기간행물)] - Lee JA / 1992 / Changes in intracellular free calcium concentration during long exposures to simulated ischemia in isolated mammalian ventricular muscle / Circ Res 71 : 58 ~ 69
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UCI(KEPA) : I410-ECN-0101-2018-524-001598274