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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 330 - 338 (9page)
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초록· 키워드
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Background: Ginsenoside Rh2 (G-Rh2) is a ginseng saponin that is widely investigated because of its remarkable antitumor activity. However, the molecular mechanism by which (20S) G-Rh2 triggers its functions and how target animals avoid its cytotoxic action remains largely unknown.
Methods: Phage display was used to screen the human targets of (20S) G-Rh2. Fluorescence spectroscopy and UV-visible absorption spectroscopy were used to confirm the interaction of candidate target proteins and (20S) G-Rh2. Molecular docking was utilized to calculate the estimated free energy of binding and to structurally visualize their interactions. MTT assay and immunoblotting were used to assess whether human serum albumin (HSA), bovine serum albumin (BSA), and bovine serum can reduce the cytotoxic activity of (20S) G-Rh2 in HepG2 cells.
Results: In phage display, (20S) G-Rh2-beads and (20R) G-Rh2-beads were combined with numerous kinds of phages, and a total of 111 different human complementary DNAs (cDNA) were identified, including HSA which had the highest rate. The binding constant and number of binding site in the interaction between (20S)-Rh2 and HSA were 3.5 x 10<SUP>5</SUP> M<SUP>-1</SUP> and 1, and those in the interaction between (20S) G-Rh2 and BSA were 1.4 x 105 M1 and 1. The quenching mechanism is static quenching. HSA, BSA and bovine serum significantly reduced the proapoptotic effect of (20S) G-Rh2.
Conclusion: HSA and BSA interact with (20S) G-Rh2. Serum inhibited the activity of (20S) G-Rh2 mainly due to the interaction between (20S) G-Rh2 and serum albumin (SA). This study proposes that HSA may enhance (20S) G-Rh2 water solubility, and thus might be used as nanoparticles in the (20S) G-Rh2 delivery process.
Methods: Phage display was used to screen the human targets of (20S) G-Rh2. Fluorescence spectroscopy and UV-visible absorption spectroscopy were used to confirm the interaction of candidate target proteins and (20S) G-Rh2. Molecular docking was utilized to calculate the estimated free energy of binding and to structurally visualize their interactions. MTT assay and immunoblotting were used to assess whether human serum albumin (HSA), bovine serum albumin (BSA), and bovine serum can reduce the cytotoxic activity of (20S) G-Rh2 in HepG2 cells.
Results: In phage display, (20S) G-Rh2-beads and (20R) G-Rh2-beads were combined with numerous kinds of phages, and a total of 111 different human complementary DNAs (cDNA) were identified, including HSA which had the highest rate. The binding constant and number of binding site in the interaction between (20S)-Rh2 and HSA were 3.5 x 10<SUP>5</SUP> M<SUP>-1</SUP> and 1, and those in the interaction between (20S) G-Rh2 and BSA were 1.4 x 105 M1 and 1. The quenching mechanism is static quenching. HSA, BSA and bovine serum significantly reduced the proapoptotic effect of (20S) G-Rh2.
Conclusion: HSA and BSA interact with (20S) G-Rh2. Serum inhibited the activity of (20S) G-Rh2 mainly due to the interaction between (20S) G-Rh2 and serum albumin (SA). This study proposes that HSA may enhance (20S) G-Rh2 water solubility, and thus might be used as nanoparticles in the (20S) G-Rh2 delivery process.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (33)
참고문헌 신청
[학술지(정기간행물)] - Yingjia Lin / 2015 / Effect of sun ginseng potentiation on epirubicin and paclitaxel-induced apoptosis in human cervical cancer cells / Journal of Ginseng Research 39 (1) : 22 ~ 28
[학술지(정기간행물)] - Kim YJ / 2015 / Biosynthesis and biotechnological production of ginsenosides / Biotechnol Adv 33 (6 Pt 1) : 717 ~ 735
[학술지(정기간행물)] - Zhang JT / 1990 / Preliminary study on antiamnestic mechanism of ginsenoside Rg1 and Rb1 / Chin Med J (Engl) 103 : 932 ~ 938
[학술지(정기간행물)] - Zhang G / 2008 / Panax ginseng ginsenoside-Rg2protects memory impairment via anti-apoptosis in a rat model with vascular dementia / J Ethnopharmacol 115 : 441 ~ 448
[학술지(정기간행물)] - Kennedy DO / 2003 / Ginseng : potential for the enhancement of cognitive performance and mood / Pharmacol Biochem Behav 75 : 687 ~ 700
[학술지(정기간행물)] - Kim YJ / 2015 / Biosynthesis and biotechnological production of ginsenosides / Biotechnol Adv 33 (6 Pt 1) : 717 ~ 735
[학술지(정기간행물)] - Zhang JT / 1990 / Preliminary study on antiamnestic mechanism of ginsenoside Rg1 and Rb1 / Chin Med J (Engl) 103 : 932 ~ 938
[학술지(정기간행물)] - Zhang G / 2008 / Panax ginseng ginsenoside-Rg2protects memory impairment via anti-apoptosis in a rat model with vascular dementia / J Ethnopharmacol 115 : 441 ~ 448
[학술지(정기간행물)] - Kennedy DO / 2003 / Ginseng : potential for the enhancement of cognitive performance and mood / Pharmacol Biochem Behav 75 : 687 ~ 700
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UCI(KEPA) : I410-ECN-0101-2018-524-001599039