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논문 기본 정보

자료유형
학술저널
저자정보
Katharine Roque (Catholic University of Daegu) GyeongDong Lim (Catholic University of Daegu) EunSeob Song (Catholic University of Daegu) Ravi Gautam (Catholic University of Daegu) JaeHee Lee (Catholic University of Daegu) YeonGyeong Kim (Catholic University of Daegu) AhRang Cho (Catholic University of Daegu) SoJung Shin (Catholic University of Daegu) ChangYul Kim (Catholic University of Daegu) HyoungAh Kim (The Catholic University of Korea) Yong Heo (Catholic University of Daegu)
저널정보
계명대학교 자연과학연구소 Quantitative Bio-Science Quantitative Bio-Science Vol.35 No.2
발행연도
2016.11
수록면
61 - 66 (6page)

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초록· 키워드

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Hazardous biochemical agents in indoor animal husbandry environments promote the occurrence of various illnesses among husbandry workers and industrial animals. The relationship between endotoxin levels in dust collected from Korean beef cattle farms and markers of bovine cellular immunity was investigated. Peripheral blood was obtained from 25 cattle from five cattle farms in Korea. Endotoxin levels present in total or respirable dust were determined by the Limulus Amebocyte Lysate Kinetic method. Cytokine production was evaluated following the stimulation of peripheral mononuclear cells with concanavalin A for 72 h in a 5% CO₂ incubator. Production of both interleukin (IL)-4 and interferon (IFN)γ was significantly higher in the high endotoxin exposure group (100.9±70.6 EU/m³) compared with the low endotoxin exposure group (17.7±18.6 EU/m³), with a lower IFNγ/IL-4 ratio in animals from the high endotoxin farms, indicating immunity skewed toward a type-2 response. The proportion of γδ T lymphocyte, important bovine immune cells involved in protection against microbial infection, was lower in cattle from the high endotoxin farms than in those from the low endotoxin farms. The numbers of white blood cells, red blood cells, lymphocytes, eosinophils, and basophils were significantly downregulated in cattle from the high endotoxin farms. Overall, these results suggest a probable negative association between dust endotoxin levels and cell-mediated immunity in Korean beef cattle.

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Abstract
Introduction
Materials and Methods
Results
Discussion
References

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