지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제12호
- 발행연도
- 2017.1
- 수록면
- 2,156 - 2,164 (9page)
이용수
초록· 키워드
오류제보하기
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression in the cytoplasm of E. coli. In this study, we achieved soluble expression of TRAIL by fusing maltose-binding protein (MBP), b’a’ domain of protein disulfide isomerase (PDIb’a’), or protein disulfide isomerase at the N-terminus of TRAIL. The TRAIL was purified using subsequent immobilized metal affinity chromatography and amylose-binding chromatography, with the tag removal using tobacco etch virus protease. Approximately 4.5 mg of pure TRAIL was produced from 125 ml flask culture with a purification yield of 71.6%.
The endotoxin level of the final product was 0.4 EU/μg, as measured by the Limulus amebocyte lysate endotoxin assay. The purified TRAIL was validated and shown to cause apoptosis of HeLa cells with an EC50 and Hill coefficient of 0.6 ± 0.03 nM and 2.41 ± 0.15, respectively. The high level of apoptosis in HeLa cells following administration of purified TRAIL indicates the significance and novelty of this method for producing high-grade and high-yield TRAIL.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Bodmer JL / 2000 / Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL / J. Biol. Chem 275 : 20632 ~ 20637
Cha SS / 1999 / Expression, purification and crystallization of recombinant human TRAIL / Acta Crystallogr. D Biol. Crystallogr 55 : 1101 ~ 1104
Cha SS / 2000 / Crystal structure of TRAIL-DR5 complex identifies a critical role of the unique frame inserti
Chandrasekaran S / 2014 / TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids / PLoS One 9 : e111487 ~
Dimberg LY / 2013 / On the TRAIL to successful cancer therapy? Predicting and counteracting resistance against TRAIL-based therapeutics / Oncogene 32 : 1341 ~ 1350
Cha SS / 1999 / Expression, purification and crystallization of recombinant human TRAIL / Acta Crystallogr. D Biol. Crystallogr 55 : 1101 ~ 1104
Cha SS / 2000 / Crystal structure of TRAIL-DR5 complex identifies a critical role of the unique frame inserti
Chandrasekaran S / 2014 / TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids / PLoS One 9 : e111487 ~
Dimberg LY / 2013 / On the TRAIL to successful cancer therapy? Predicting and counteracting resistance against TRAIL-based therapeutics / Oncogene 32 : 1341 ~ 1350
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
TRAIL 저항성 인간 방광암 세포에서 histone deacetylase 억제제인 Trichostatin A에 의한 TRAIL 매개 세포 사멸 유도의 증가
생명과학회지
2025 .03
-
인체 방광암세포에서 histone deacetylase 억제제인 sodium butyrate이 TRAIL에 의한 apoptosis 유도에 미치는 영향
생명과학회지
2016 .04
-
MiR-339 attenuates LPS-induced intestinal epithelial cells inflammatory responses and apoptosis by targeting TLR4
Genes & Genomics
2020 .01
-
Suppression of neointimal hyperplasia induced by arteriovenous anastomosis and balloon injury in rats by multimeric tumor necrosis factor-related apoptosis-inducing ligand
Molecules and Cells
2024 .01
-
폐암 세포 사멸을 위한 프로테아좀 억제제와 트레일 단백질의 혼용 효과 연구
생명과학회지
2025 .02
-
칠갑산도립공원 탐방로의 물리적 특성 및 훼손유형 분석
국립공원연구지
2022 .11
-
Orostachys japonicus induce caspase-dependent apoptosis in HeLa human cervical cancer cells
Nutrition Research and Practice
2025 .02
-
Orostachys japonicus induce caspase-dependent apoptosis in HeLa human cervical cancer cells
Nutrition Research and Practice
2025 .02
-
장내세균 5종의 Endotoxin 정량 비교
대한임상검사과학회지
2016 .01
-
H9 Induces Apoptosis via the Intrinsic Pathway in Non-Small-Cell Lung Cancer A549 Cells
Journal of Microbiology and Biotechnology
2015 .01
-
운문산군립공원의 탐방로 훼손실태 분석
국립공원연구지
2021 .11
-
(E)-2-Methoxy-4-(3-(4-Methoxyphenyl)Prop-1-en-1-yl)Phenol Induces Apoptosis in HeLa Cervical Cancer Cells via the Extrinsic Apoptotic Pathway
Journal of Microbiology and Biotechnology
2017 .07
-
Picropodophyllotoxin Induces G1 Cell Cycle Arrest and Apoptosis in Human Colorectal Cancer Cells via ROS Generation and Activation of p38 MAPK Signaling Pathway
Journal of Microbiology and Biotechnology
2021 .12
-
경주국립공원 남산 지구의 탐방로 훼손 유형 및 환경피해도 평가
한국환경과학회지
2015 .11
-
S-allylcysteine의 자궁경부암세포주 HeLa에 대한 세포증식 억제효과
생명과학회지
2015 .04
-
Association of Bovine Cellular Immunity with Endotoxin Level in Dust from Korean Beef Cattle Housing Environments
Quantitative Bio-Science
2016 .11
-
Exosome extraction from conditioned media of HeLa cell using high speed centrifuge
한국분석과학회 학술대회
2023 .05
-
Small molecule NMD and MDM2 inhibitors synergistically trigger apoptosis in HeLa cells
Molecules and Cells
2024 .01
-
Polysiponia morrowii Extract Inhibits Cancer Growth on CT-26 and Hela cells
한국해양바이오학회지
2020 .12
-
도보여행 활성화를 위한 탐방로 시설 및 운영 관리 실태 평가- 강화 나들길을 중심으로 -
한국습지학회지
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0