지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 49 - 58 (10page)
이용수
초록· 키워드
오류제보하기
Cell permeable peptide (CPP) is able to transport itself or conjugated molecules such as nucleotides, peptides, and proteins into cells. Since short peptide of human immunodeficiency virus-1 Tat has been discovered as CPP, it has been continuously studied for their ability to transport heterologous cargoes into cells. In this study, we have focused on the fusion protein of respiratory syncytial virus (RSV), which has six basic amino acids in multi basic furin-dependent cleavage site (MBFCS) required to be cationic CPP. To develop more efficient CPP, the sequence, which linked two MBFCS, was synthesized (called RS-CPP). To assess cell permeable efficiency of RSCPP or MBFCS, the peptides was conjugated with fluorescein isothiocyanate, and cell permeable efficiency was measured by fluorescence-activated cell sorting. Cell permeability of RS-CPP or MBFCS was increased in a dose-dependent manner, but RS-CPP showed more efficient cell permeability than MBFCS in MDCK, HeLa, Vero E6, and A549 cells. To evaluate whether RS-CPP can transport its conjugated functional peptide (VIVIT) in CD8+ T cell, it was confirmed that IL-2 and β-galactosidase expression were significantly inhibited through selective block of nuclear factor activated T-cell. To investigate endocytic pathways, Cre-mediated DNA recombination (loxP-STOP-loxP-LacZ reporter system) was investigated with divergent endocytosis inhibitors in TE671 cells, and RS-CPP endocytosis is occurred via binding cell surface glycosaminoglycan and clathrin-mediated endocytosis, or macropinocytosis. These results indicated that RS-CPP could be a novel cationic CPP, and it would help understanding for delivery of biologically functional molecules based on viral basic amino acids.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (24)
참고문헌 신청
[학술지(정기간행물)] - Heitz F / 2009 / Twenty years of cell-penetrating peptides: from molecular mechanisms to therapeutics / Br J Pharmacol 157 : 195 ~ 206
[학술지(정기간행물)] - Milletti F / 2012 / Cell-penetrating peptides: classes, origin, and current landscape / Drug Discov Today 17 : 850 ~ 860
[학술지(정기간행물)] - Mae M / 2006 / Cell-penetrating peptides as vectors for peptide, protein and oligonucleotide delivery / Curr Opin Pharmacol 6 : 509 ~ 514
[학술지(정기간행물)] - Rizzuti M / 2015 / Therapeutic applications of the cell-penetrating HIV-1 Tat peptide / Drug Discov Today 20 : 76 ~ 85
[학술지(정기간행물)] - Wadia JS / 2004 / Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis / Nat Med 10 : 310 ~ 315
[학술지(정기간행물)] - Milletti F / 2012 / Cell-penetrating peptides: classes, origin, and current landscape / Drug Discov Today 17 : 850 ~ 860
[학술지(정기간행물)] - Mae M / 2006 / Cell-penetrating peptides as vectors for peptide, protein and oligonucleotide delivery / Curr Opin Pharmacol 6 : 509 ~ 514
[학술지(정기간행물)] - Rizzuti M / 2015 / Therapeutic applications of the cell-penetrating HIV-1 Tat peptide / Drug Discov Today 20 : 76 ~ 85
[학술지(정기간행물)] - Wadia JS / 2004 / Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis / Nat Med 10 : 310 ~ 315
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UCI(KEPA) : I410-ECN-0101-2018-475-003113833