지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 447 - 454 (8page)
이용수
초록· 키워드
오류제보하기
Background: Korean ginseng has been widely evaluated to treat human diseases; however, most studies on Korean ginseng have focused on its root. In this study, polysaccharides [acidic-polysaccharide-linked glycopeptide (APGP) extracted with 90% ethanol and hot water] were prepared from Korean ginseng berries, and their effect on immunosenescence was explored.
Methods: The effect of APGP on thymic involution was evaluated by measuring the size of thymi dissected from aged mice. The effect of APGP on populations of immune cells, including natural killer (NK) cells, dendritic cells, age-correlated CD11c-positive B cells, and several subtypes of T cells [CD4-positive, CD8-positive, and regulatory (Treg) T cells] in the thymi and spleens of aged mice was analyzed by fluorescence-activated cell sorting analysis. Serum levels of interleukin (IL)-2 and IL-6 were evaluated by enzyme-linked immunosorbent assay analysis. Profiles of APGP components were evaluated by high-performance liquid chromatography (HPLC) analysis.
Results: APGP suppressed thymic involution by increasing the weight and areas of thymi in aged mice. APGP increased the population of NK cells, but showed no effect on the population of dendritic cells in the thymi and spleens of aged mice. APGP decreased the population of age-correlated CD11c-positive B cells in the spleens of aged mice. APGP showed no effect on the populations of CD4- and CD8-positive T cells in the thymi of aged mice, whereas it increased the population of Treg cells in the spleens of aged mice. APGP further decreased the reduced serum levels of IL-2 in aged mice, but serum levels of IL-6 were not statistically changed by APGP in aged mice. Finally, HPLC analysis showed that APGP had one major peak at 15 min (a main type of polysaccharide) and a long tail up to 35 min (a mixture of a variety of types of polysaccharides).
Conclusion: These results suggested that APGP exerted an anti-immunosenescent effect by suppressing thymic involution and modulating several types of immune cells.
Methods: The effect of APGP on thymic involution was evaluated by measuring the size of thymi dissected from aged mice. The effect of APGP on populations of immune cells, including natural killer (NK) cells, dendritic cells, age-correlated CD11c-positive B cells, and several subtypes of T cells [CD4-positive, CD8-positive, and regulatory (Treg) T cells] in the thymi and spleens of aged mice was analyzed by fluorescence-activated cell sorting analysis. Serum levels of interleukin (IL)-2 and IL-6 were evaluated by enzyme-linked immunosorbent assay analysis. Profiles of APGP components were evaluated by high-performance liquid chromatography (HPLC) analysis.
Results: APGP suppressed thymic involution by increasing the weight and areas of thymi in aged mice. APGP increased the population of NK cells, but showed no effect on the population of dendritic cells in the thymi and spleens of aged mice. APGP decreased the population of age-correlated CD11c-positive B cells in the spleens of aged mice. APGP showed no effect on the populations of CD4- and CD8-positive T cells in the thymi of aged mice, whereas it increased the population of Treg cells in the spleens of aged mice. APGP further decreased the reduced serum levels of IL-2 in aged mice, but serum levels of IL-6 were not statistically changed by APGP in aged mice. Finally, HPLC analysis showed that APGP had one major peak at 15 min (a main type of polysaccharide) and a long tail up to 35 min (a mixture of a variety of types of polysaccharides).
Conclusion: These results suggested that APGP exerted an anti-immunosenescent effect by suppressing thymic involution and modulating several types of immune cells.
목차
abstract
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (47)
참고문헌 신청
[학술지(정기간행물)] - Lipsky MS / 2015 / Biological theories of aging / Dis Mon 61 : 460 ~ 466
[학술지(정기간행물)] - Robert L / 2014 / Aging. Facts and theories. Preface / Interdiscip Top Gerontol 39 : VI ~ VIII
[학술지(정기간행물)] - Lopez-Otin C / 2013 / The hallmarks of aging / Cell 153 : 1194 ~ 1217
[학술지(정기간행물)] - Solana R / 2012 / Innate immunosenescence: effect of aging on cells and receptors of the innate immune system in humans / Semin Immunol 24 : 331 ~ 341
[학술지(정기간행물)] - Montgomery RR / 2015 / Paradoxical changes in innate immunity in aging: recent progress and new directions / J Leukoc Biol 98 : 937 ~ 943
[학술지(정기간행물)] - Robert L / 2014 / Aging. Facts and theories. Preface / Interdiscip Top Gerontol 39 : VI ~ VIII
[학술지(정기간행물)] - Lopez-Otin C / 2013 / The hallmarks of aging / Cell 153 : 1194 ~ 1217
[학술지(정기간행물)] - Solana R / 2012 / Innate immunosenescence: effect of aging on cells and receptors of the innate immune system in humans / Semin Immunol 24 : 331 ~ 341
[학술지(정기간행물)] - Montgomery RR / 2015 / Paradoxical changes in innate immunity in aging: recent progress and new directions / J Leukoc Biol 98 : 937 ~ 943
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Upregulation of CD47 in Regulatory T Cells in Atopic Dermatitis
Yonsei Medical Journal
2016 .01
-
Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Out-sourcing for Trans-presentation : Assessing T Cell Intrinsic and Extrinsic IL-15 Expression with Il15 Gene Reporter Mice
Immune Network
2018 .02
-
Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil
Journal of Ginseng Research
2020 .03
-
Immunological and pathological analyses of peripheral blood mononuclear cell-engraft humanized mouse using NOD-Rag2null IL2rgnull mice
한국실험동물학회 학술발표대회 논문집
2021 .01
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
Regulation of Th2 Cell Immunity by Dendritic Cells
Immune Network
2016 .02
-
Cytotoxicity of natural killer cells co-cultured with γ-irradiatedor mitomycin C-treated feeder cells
예방수의학회지
2020 .01
-
CD47 deficient mice exhibit enhanced human peripheral blood mononuclear cell engraftment in NOD-Rag2null IL2rgnull condition
한국실험동물학회 학술발표대회 논문집
2021 .07
-
Interleukin-10-Producing B Cells Help Suppress Ovariectomy-Mediated Osteoporosis
Immune Network
2020 .12
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
IL-17-Producing Cells in Tumor Immunity: Friends or Foes?
Immune Network
2020 .02
-
Effect of IL-4 on the Development and Function of Memory-like CD8 T Cells in the Peripheral Lymphoid Tissues
Immune Network
2016 .04
-
Clonal Expansion of Allergen-specific CD4+ T Cell in the Lung in the Absence of Lymph Nodes
Immune Network
2017 .06
-
Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice
Parasites, Hosts and Diseases
2016 .10
-
Optimization of Large-Scale Expansion and Cryopreservation of Human Natural Killer Cells for Anti-Tumor Therapy
Immune Network
2018 .08
-
Re-defining T-Cell Exhaustion: Subset, Function, and Regulation
Immune Network
2020 .02
-
Immunological benefits by ginseng through reciprocal regulation of Th17 and Treg cells during cyclosporine-induced immunosuppression
Journal of Ginseng Research
2016 .01
-
The increasing hematopoietic effect of the combined treatment of Korean Red Ginseng and Colla corii asini on cyclophosphamide-induced immunosuppression in mice
Journal of Ginseng Research
2021 .09
-
Th17 Cell and Inflammatory Infiltrate Interactions in Cutaneous Leishmaniasis: Unraveling Immunopathogenic Mechanisms
Immune Network
2024 .04
이 논문의 저자 정보
이 논문과 함께 이용한 논문
Effect of Polysaccharides from a Korean Red Ginseng Berry on the Immunosenescence of Aged Mice
Mi Seon Kim
,
Yohan Hong
,
Eun Ji Kim
외 8명
한국약용작물학회 학술대회논문집
2019
.10
Aβ42로 유도된 알츠하이머 마우스 모델에서 이중 가공 인삼열매 추출물의 학습 및 기억 손실 개선 효과
장수길
,
안정원
,
조보람
외 7명
한국식품과학회지
2019
.04
Efficacy and safety of Panax ginseng berry extract on glycemic control : A 12-wk randomized, double-blind, and placebo-controlled clinical trial
Han Seok Choi
,
Sunmi Kim
,
Min Jung Kim
외 7명
Journal of Ginseng Research
2018
.01
D-galactosamine/lipopolysaccharide로 감작된 급성간독성 마우스 모델에서 인삼열매추출물의 간독성 개선 효과
장수길
,
박준섭
,
안정원
외 8명
한국식품과학회지
2017
.12
생체친화성 균주에 의한 인삼열매증포 추출물의 최적발효조건 및 항산화활성
김승태
,
김희정
,
장수길
외 2명
한국식품과학회지
2013
.02
최근 본 자료
댓글(0)
0