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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 283 - 295 (13page)
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초록· 키워드
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Objectives
In this study, we characterized human dental pulp cells (HDPCs) obtained by different culture methods to establish the most suitable methodology for dental tissue engineering and regenerative endodontic applications.
Materials and Methods
HDPCs were isolated by the outgrowth method (HDPCs-OG), the enzymatic digestion method (collagenase/dispase/trypsin, HDPCs-ED), or the combination of both methods (HDPCs-Combined). The expression of mesenchymal stem cell markers (CD105, CD90, and CD73) was investigated. In vitro differentiation capacities of HDPCs into adipogenic, osteogenic, and chondrogenic lineages were compared. Differentiation markers were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blotting.
Results
Our data indicated that whole HDPCs-ED, HPDCs-OG, and HDPCs-Combined could be differentiated into adipogenic, chrondrogenic, and osteogenic cell types. However, we found that the methods for isolating and culturing HDPCs influence the differentiation capacities of cells. HDPCs-OG and HDPCs-ED were preferably differentiated into adipogenic and osteogenic cells, respectively. Differentiation markers shown by RT-PCR and western blotting analysis were mostly upregulated in the treated groups compared with the control groups.
Conclusions
Our findings confirmed that cell populations formed by two different culture methods and the combined culture method exhibited different properties. The results of this study could provide an insight into regenerative endodontic treatment using HDPCs.
목차
등록된 정보가 없습니다.
참고문헌 (37)
참고문헌 신청
Miura M / 2003 / SHED: stem cells from human exfoliated deciduous teeth / Proc Natl Acad Sci USA 100:5807~5812
Malhotra N / 2012 / Regenerative endodontics as a tissue engineering approach: past, current, and future / Aust Endod J 38:137~148
About I / 2000 / Human dentin production in vitro / Exp Cell Res 258:33~41
Huang GT / 2009 / Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine / J Dent Res 88:792~806
Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci USA 97:13625~13630
Malhotra N / 2012 / Regenerative endodontics as a tissue engineering approach: past, current, and future / Aust Endod J 38:137~148
About I / 2000 / Human dentin production in vitro / Exp Cell Res 258:33~41
Huang GT / 2009 / Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine / J Dent Res 88:792~806
Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci USA 97:13625~13630
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