지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 318 - 333 (16page)
이용수
초록· 키워드
오류제보하기
Objectives : In this study, the author tried to examine whether Cheogjogupye-tang (淸燥救肺湯, CGPT) and Yieum-jeon (理陰煎, YEJ) significantly affect in vitro and in vivo mucin secretion, MUC5AC gene expression in airway epithelial cells and contractility of isolated tracheal smooth muscle of rabbit.
Materials and Methods : For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were chased for 30 minutes in the presence of CGPT and YEJ to assess the effects of the agents on mucin secretion by enzyme-linked immunosorbent assay (ELISA), with removal of oriental herbal medicine extract from each agent-treated sample by centrifuge microfilter. Also, the effects of the agents on TNF-alpha or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agent were assessed by examining both LDH release from HTSE cells and the rate of survival and proliferation of NCI-H292 cells. For in vivo experiment, hypersecretion of airway mucin and goblet cell hyperplasia was induced by exposure of rats to SO2 over 3 weeks. Effects of CGPT and YEJ orally administered for 1 week on in vivo mucin secretion from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using ELISA and histological analysis after staining the epithelial tissue with alcian blue, respectively. Also, the effects of CGPT and YEJ on contractility of isolated tracheal smooth muscle were investigated.
Results : (1) CGPT significantly inhibited in vitro mucin secretion from cultured HTSE cells. However, YEJ did not affect in vitro mucin secretion; (2) CGPT and YEJ did not affect hypersecretion of in vivo mucin and hyperplasia of tracheal goblet cells; (3) CGPT and YEJ slightly increased the expression levels of TNF-alpha or EGF-induced MUC5AC gene in NCI-H292 cells; (4) CGPT and YEJ inhibited acetylcholine-induced contraction of isolated tracheal smooth muscle of rabbit; (5) CGPT and YEJ did not affect LDH release from HTSE cells and the survival and proliferation of NCI-H292 cells.
Conclusion : The results from the present study suggest that CGPT and YEJ mainly affect the expression of mucin gene rather than secretion of mucin and do not show remarkable cytotoxicity to respiratory epithelial cells.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Culpitt SV / 2005 / Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma / Respir Med 99 (6) : 703 ~ 710
Frigas E / 1981 / Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma / Mayo Clin Proc 56 : 345 ~ 353
Gleich GJ / 1990 / The eosinophil and bronchial asthma: Current understanding / Journal Allergy Clin Immunol 85 : 422 ~ 436
Harkema JR / 1992 / In vivo effects of endotoxin on intraepithelial mucosubstances in rat pulmonary airways: quantitative histochemistry / Am J Pathol 141 : 307 ~ 331
Karlinsey J / 1989 / Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells / Anal Biochem 180 (2) : 303 ~ 306
Frigas E / 1981 / Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma / Mayo Clin Proc 56 : 345 ~ 353
Gleich GJ / 1990 / The eosinophil and bronchial asthma: Current understanding / Journal Allergy Clin Immunol 85 : 422 ~ 436
Harkema JR / 1992 / In vivo effects of endotoxin on intraepithelial mucosubstances in rat pulmonary airways: quantitative histochemistry / Am J Pathol 141 : 307 ~ 331
Karlinsey J / 1989 / Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells / Anal Biochem 180 (2) : 303 ~ 306
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