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Background and Objectives:Mucin secretion is regulated by the mucin genes (MUC) in the respiratory, gastrointestinal and reproductive system. Inflammation induces mucin hypersecretion in the human body. This study demonstrates the efects of IL-1βMaterials and Method:Analysis of MUC2 gene was done by RT-PCR and the protein analysis was done by a flow cyto-metric analysis and an immunoasay method using cultured human airway epithelial cells, and NCI-H292 cells. Results:The expresion of MUC2 mRNA and protein induced by IL-1β increased in a dose-and time-dependent maner. The maxi-mum mRNA level of the MUC2 gene was approximately 3-fold, compared to that of the control cell. The IL-1β-mediated MUC2 protein started at 6 hours of exposure to IL-1β (20 ng/ml) and the maximum level was 12 hours. The MUC2 protein data of flow cytometric analysis coresponded to that of immunoassay analysis. The expresion of MUC2 gene was suppr-esed by actinomycin D, but not attenuated by cycloheximide. Conclusion:These results suggest that the IL-1β-mediated MUC2 gene and protein expresion were increased in a dose- and time-dependent pattern and regulated by transcriptional step. (Korean J Otolaryngol 2002;45:35-40)

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