백서의 이관에서 Water Channel Protein(Aquaporin)들의분포와 발현에 관한 연구

진정언; 이창훈; 정민섭; 김형곤; 김보형; 오대현; 강성호; 임대준

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자료유형: 학술저널

저자정보: 진정언 이창훈 정민섭 김형곤 김보형 오대현 강성호 임대준

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제6호

발행연도: 2006.1

수록면: 598 - 603 (6page)

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Background and Objectives：The mucosal epithelium of middle ear and eustachian tube needs hydration to maintain properamount of moisture. Recent discoveries of various types of water channel proteins (aquaporins；AQPs) in mucous epitheliumsuggest that they play critical roles in the regional regulation of water balance. The purpose of this study is to assess the distributionand expression of different subtypes of AQPs in the rat eustachian tube. Subjects and Method：We analyzed the distributionand expression of AQP1, 2, 4, 5, and 7 using RT-PCR, Western blot, and immunohistochemistry study in the rat middle ear andeustachian tube samples. Results：The expression of the mRNA of AQP1, 4, and 5 were observed in the rat eustachian tube andmiddle ear. The bands of AQP1, 4, and 5 were detected at 28 kDa, 31 kDa, and 26 kDa proteins in the Western blot analysis,respectively. The immunohistochemical analysis demonstrated that AQP1 was expressed in fibroblasts at subepithelial portionsof the rat eustachian tube and that AQP4 was observed at the basolateral membrane of the ciliated epithelial cells. The distributionof AQP5 in rat eustachian tube was limited to the apical surface of serous gland cells, but all of the epithelial goblet cells werelabeled negative. Conclusion：We demonstrated that there are various subtypes of AQP in rat eustachian tube and that they existedat specific cells and sites. This study may be a preliminary study to determine the possibility of relationship between AQP expressionand AQP-related disease in the middle ear and eustachian tube. (Korean J Otolaryngol 2006;49:598-603)

#Aquaporin #AQP)·이관·역전사효소 중합효소연쇄반응·면역조직화학염색·Western blotting. #Aquaporins·Eustachian tube·Reverse transcriptase polymerase chain reaction·Immunohistochemistry·Western blotting.

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