수분 제한 기니픽에서 내이의 Aquaporin-2 발현 변화

이성수; 김각; 김정현; 조형호; 장철호; 조용범

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자료유형: 학술저널

저자정보: 이성수 김각 김정현 조형호 장철호 조용범

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제52권 제7호

발행연도: 2009.1

수록면: 572 - 578 (7page)

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Background and Objectives Although the role of aquaporin-2 (AQP2) in the kidney has been well defined, its role in the inner ear remains to be determined. The present study was to investigate the effect of water deprivation on the expression of AQP2 in the inner ear. Materials and Method Healthy male guinea pigs weighing 250 g were used. The experimental group underwent water restriction and the control underwent water loading with sucrose-containing water for 3 days. Concentrations of plasma arginine-vasopressin (AVP) were determined and electrocochleography (ECoG) recordings were made. An RT-PCR, real-time PCR and Westernblotting analysis were used for quantitative analysis of AQP2 mRNA and AQP2 protein expression. Immunohistochemistry was also used to evaluate the distribution of AQP2 water channel proteins in the inner ear. Results AQP2 was mainly expressed in the epithelium of endolymphatic sac, spiral limbus, spiral ligament and stria vascularis of scala media. The concentrations of plasma AVP were 9.2± 0.8 pg/mL in the experimental group and 0.78±0.3 pg/mL in the control. The summation potential/ action potential (SP/AP) ratio in ECoG was markedly increased in the experimental group (0.55 in the experimental and 0.29 in the control). RT-PCR and real time PCR as well as Western blot analysis showed that the level of AQP2 mRNA and protein in the cochlea and endolymphactic sac of the water-deprived group was significantly higher than those in the control group. Conclusion These data suggest that AQP2 is one of the important water channels in fluid homeostasis in the inner ear. Moreover, the volume of endolymphatic space can be increased via AVP-AQP2 system in response to water deprivation.

#Aquaporin 2·Endolymphatic hydrops·Arginine vasopressin·Inner ear

참고문헌 (20)

참고문헌 신청

Beitz E / 1999 / Expression pattern of aquaporin water channels in the inner ear of the rat. The molecular basis for a water regulation system in the endolymphatic sac / Hear

Beitz E / 2003 / Aquaporin-mediated fluid regulation in the inner ear / Cell Mol Neurobiol 23 (3) : 315 ~ 329

Breyer MD / 1994 / Hormonal signaling and regulation of salt and water tranport in the collecting duct / Annu Rev Physiol (56) : 711 ~ 739

Couloigner V / 2004 / Aquaporin-2 in the Human Endolymphatic Sac / Acta Otolaryngol 124 (4) : 449 ~ 453

Gu FM / 2006 / Effects of vasopressin on gene expression in rat inner ear / Hear Res 222 (1) : 70 ~ 78

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