자가골수줄기세포를 이용한 골 형성 임플란트의 개발

박동준; 김현수; 양종원; 김성일; 서정옥

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자료유형: 학술저널

저자정보: 박동준 김현수 양종원 김성일 서정옥

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제49권 제3호

발행연도: 2006.1

수록면: 297 - 302 (6page)

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Background and Objectives：The aim of the current study was to examine ectopic bone formation with autologous mesenchymal stem cells (MSC) on a porous 3-D Poly (DL-lactic-co-glycolic acid, PLGA) in canine model. Materials and Method：Bone marrow derived autologous mesenchymal stem cells (MSCs) were isolated and purified from dogs. The porous PLGA scaffold loaded MSCs was treated with osteo-inductive medium containing dexamethasone, beta glycerophosphate and ascorbic acid. Osteogenic differentiation and mineralization were examined with RT-PCR for osteocalcin mRNA and Von Kossa staining of the cell-polymer complex. For in vivo bone formation, polymers containing osteogenic differentiated cells were implanted into the subperiosteal space of the canine maxillary sinus and evaluated at 1st and 2nd month. Results：In vitro osteogenic differentiation was evident from 2 weeks after osteogenic induction. The mineralization was increased in a time dependent manner. Ectopic bone was generated in the subperiosteal space of canine maxillary sinus at 1 month and matured at 2 months. Conclusion：From these results, we conclude that ectopic tissue engineered bone formation in the facial area is possible and that it may be useful for facial reconstruction or regeneration of bone defect. (Korean J Otolaryngol 2006;49:297-302)

#조직공학·간엽줄기세포·골분화·세포분화. #Tissue engineering·Mesenchymal Stem cells·Osteogenesis·Cell differentiation.

참고문헌 (14)

참고문헌 신청

/ 1996 / De novo cartilage generation using calcium alginate-chondrocyteconstructs 97 : 168 ~ 178

/ 1997 / Culture expandedcanine mesenchymal stem cells posess osteochondrogenic potential invivo and in vitro 6 : 125 ~ 134

/ 1997 / Ectopic bone formation by marrow stromal osteoblasttransplantation using poly foams implantedinto the rat mesentery 36 : 1 ~ 8

/ 1997 / Osteogenic differentiationof purified, culture-expanded human mesenchymal stem cellsin vitro 64 : 295 ~ 312

/ 1998 / New approach by tissue engineering for extended selective transplantationwith a pancreatic B-cell line 30 : 83 ~ 85

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