Neural-Induced Human Mesenchymal Stem Cells Promote Cochlear Cell Regeneration in Deaf Guinea Pigs

조용범; 장수정; 김송희; 박종성; 정한성; 조형호; 이경화; 전재열

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자료유형: 학술저널

저자정보: 조용범 장수정 김송희 박종성 정한성 조형호 이경화 전재열

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제8권 제2호

발행연도: 2015.1

수록면: 83 - 91 (9page)

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Objectives. In mammals, cochlear hair cell loss is irreversible and may result in a permanent sensorineural hearing loss. Secondary to this hair cell loss, a progressive loss of spiral ganglion neurons (SGNs) is presented. In this study, we have investigated the effects of neural-induced human mesenchymal stem cells (NI-hMSCs) from human bone marrow on sensory neuronal regeneration from neomycin treated deafened guinea pig cochleae. Methods. HMSCs were isolated from the bone marrow which was obtained from the mastoid process during mastoidectomy for ear surgery. Following neural induction with basic fibroblast growth factor and forskolin, we studied the several neural marker and performed electrophysiological analysis. NI-hMSCs were transplanted into the neomycin treated deafened guinea pig cochlea. Engraftment of NI-hMSCs was evaluated immunohistologically at 8 weeks after transplantation. Results. Following neural differentiation, hMSCs expressed high levels of neural markers, ionic channel markers, which are important in neural function, and tetrodotoxin-sensitive voltage-dependent sodium currents. After transplantation into the scala tympani of damaged cochlea, NI-hMSCs-injected animals exhibited a significant increase in the number of SGNs compared to Hanks balanced salt solution-injected animals. Transplanted NI-hMSCs were found within the perilymphatic space, the organ of Corti, along the cochlear nerve fibers, and in the spiral ganglion. Furthermore, the grafted NI-hMSCs migrated into the spiral ganglion where they expressed the neuron-specific marker, NeuN. Conclusion. The results show the potential of NI-hMSCs to give rise to replace the lost cochlear cells in hearing loss mammals.

#Hair Cells #Auditory #Mesenchymal Stem Cells #Hearing Loss #Cell Differentiation #Transplantation

참고문헌 (30)

참고문헌 신청

Sharif S / 2007 / The potential use of bone marrow stromal cells for cochlear cell therapy / Neuroreport 18(4):351~354

Kamiya K / 2007 / Mesenchymal stem cell transplantation accelerates hearing recovery through the repair of injured cochlear fibrocytes / Am J Pathol 171(1):214~226

Matsuoka AJ / 2007 / Enhanced survival of bone-marrow-derived pluripotent stem cells in an animal model of auditory neuropathy / Laryngoscope 117(9):1629~1635

Hu Z / 2005 / Survival and neural differentiation of adult neural stem cells transplanted into the mature inner ear / Exp Cell Res 302(1):40~47

Chen X / 2002 / Ischemic rat brain extracts induce human marrow stromal cell growth factor production / Neuropathology 22(4):275~279

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