지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1 - 13 (13page)
이용수
초록· 키워드
오류제보하기
Clinical expansion of mesenchymal stem cells (MSCs) is hampered by the lack of knowledge regarding how to prevent MSC apoptosis and promote their proliferation in serum-free medium. Our in vitro studies demonstrated that human umbilical cord MSCs (HUCMSCs) underwent apoptosis in the serum-free medium. When HUCMSCs were co-cultured with retinal pigment epithelial cells (ARPE19), however, HUCMSCs exhibited normal growth and morphology in serum-free medium. Their colony formation was promoted by the conditioned medium (CM) of ARPE19 cells on Matrigel. Proteomics analysis showed that pigment epithelium-derived factor (PEDF) was one of the most abundant extracellular proteins in the ARPE19 CM, whereas enzyme-linked immunosorbent assay confirmed that large amounts of PEDF was secreted from ARPE19 cells. Adding anti-PEDFblocking antibodies to the co-culture of HUCMSCs with ARPE19 cells increased apoptosis of HUCMSCs. Conversely, treatment with PEDF significantly reduced apoptosis and increased proliferation of HUCMSCs in serum-free medium. PEDF was further demonstrated to exert this anti-apoptotic effect by inhibiting P53 expression to suppress caspase activation. In vivo studies demonstrated that co-injection of HUCMSCs with ARPE19 cells in immunocompromised NOD-SCID mice also increased survival and decreased apoptosis of HUCMSCs. PEDF also showed no negative effect on the mesoderm differentiation capability of HUCMSCs. In conclusion, this study is the first to demonstrate that PEDF promotes HUCMSC proliferation and protects them from apoptosis by reducing p53 expression in the serum-free medium. This study provides crucial information for clinical-scale expansion of HUCMSCs.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Di Lauro S / 2016 / A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells / Mol Vis 22 : 243 ~ 253
Ding DC / 2007 / Enhancement of neuroplasticity through upregulation of beta1-integrin in human umbilical cord-derived stromal cell implanted stroke model / Neurobiol Dis 27
Ding DC / 2015 / Human umbilical cord mesenchymal stem cells : a new era for stem cell therapy / Cell Transplant 24 : 339 ~ 347
Ding DC / 2016 / Characterization of HLA-G and related immunosuppressive effects in human umbilical cord stroma-derived stem cells / Cell Transplant 25 : 217 ~ 228
Duan P / 2013 / Human bone marrow stromal cells can differentiate to a retinal pigment epithelial phenotype when co-cultured with pig retinal pigment epithelium using a tran
Ding DC / 2007 / Enhancement of neuroplasticity through upregulation of beta1-integrin in human umbilical cord-derived stromal cell implanted stroke model / Neurobiol Dis 27
Ding DC / 2015 / Human umbilical cord mesenchymal stem cells : a new era for stem cell therapy / Cell Transplant 24 : 339 ~ 347
Ding DC / 2016 / Characterization of HLA-G and related immunosuppressive effects in human umbilical cord stroma-derived stem cells / Cell Transplant 25 : 217 ~ 228
Duan P / 2013 / Human bone marrow stromal cells can differentiate to a retinal pigment epithelial phenotype when co-cultured with pig retinal pigment epithelium using a tran
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells
조직공학과 재생의학
2023 .06
-
A Comprehensive Cancer-Associated MicroRNA Expression Profiling and Proteomic Analysis of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes
조직공학과 재생의학
2022 .10
-
Angle-resolved photoemission spectroscopy for the study of two-dimensional materials
Nano Convergence
2017 .03
-
Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application
조직공학과 재생의학
2021 .01
-
Development of Serum-Free Media for Adipose-derived Mesenchymal Stem Cells Using Design of Experiments
한국생물공학회 학술대회
2022 .09
-
Three-Dimensional Culture of Mesenchymal Stem Cells
조직공학과 재생의학
2015 .01
-
Inhibition of apoptosis by silkworm storage protein 1 in HeLa cell
한국생물공학회 학술대회
2015 .04
-
Apoptotic cell-derived exosomes: messages from dying cells
Experimental and Molecular Medicine
2020 .01
-
Superoxide dismutase 3 protects mesenchymal stem cells through enhanced autophagy and regulation of FoxO3a trafficking
BMB Reports
2018 .01
-
Coculture Effects on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells
조직공학과 재생의학
2016 .01
-
Glutathione peroxidase-1 regulates ASK1-dependent apoptosis via interaction with TRAF2 in RIPK3-negative cancer cells
Experimental and Molecular Medicine
2021 .06
-
Effect of Low Temperature on Schwann-Like Cell Differentiation of Bone Marrow Mesenchymal Stem Cells
조직공학과 재생의학
2015 .01
-
Annexin A2 as a regulator of the cellular senescence and apoptosis through p21
한국생물공학회 학술대회
2015 .04
-
Umbilical cord mesenchymal stem cell-conditioned medium improves palmitate-induced insulin resistance in C2C12 myotubes
한국생물공학회 학술대회
2019 .04
-
The promotive effect of human induced pluripotent stem cell-conditioned medium on growth and migration of human skin cells
한국생물공학회 학술대회
2016 .10
-
Isolation of Cabbage Exosome-like Nanovesicles and Investigation of Their Biological Activities in Human Cells
한국생물공학회 학술대회
2021 .04
-
Half-Serum DMEM: Revolutionizing Cell Culture Medium to Reduce FBS Dependency, Costs, and Environmental Impact
한국생물공학회 학술대회
2024 .09
-
Hypoxia Enhances Cell Properties of Human Mesenchymal Stem Cells
조직공학과 재생의학
2017 .01
-
Bolstering the secretion and bioactivities of umbilical cord MSC-derived extracellular vesicles with 3D culture and priming in chemically defined media
Nano Convergence
2022 .12
-
Immunomodulatory effect of mesenchymal stem cells and mesenchymal stem-cell-derived exosomes for COVID-19 treatment
BMB Reports
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0