지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 591 - 598 (8page)
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초록· 키워드
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In vitro large amplification of tumor-specific cyto-toxic T lymphocytes (CTLs) and adoptive transfer of these cells is one of the most promising approaches to treat malignant diseases in which an effective immune response is not achieved by active immuni-zation. However, generating sufficient numbers of tumor-specific CTLs stimulated with autologous antigen presenting cells (APCs) in vitro is one of the most problematic steps in the adoptive cell transfer (ACT) therapy. To circumvent this problem, we have developed an artificial antigen presenting complex (aAPCs) using MHC class I molecules loaded with a sults show that TRP-2-specific CD8+ T cells elicited by immunization with recombinant adenovirus expressing the mini-gene epitope are efficiently stimulated and amplified in vitro to a greater extent by aAPCs than by natural splenic APCs. These aAPC-induced CTLs recognized endogenously pro-cessed antigens present on B16F10 melanoma cells. Efficient stimulation and proliferation of antigen- specific T cells was also confirmed using ovalbumin peptide-loaded aAPCs and OT-I TCR transgenic cells. These results demonstrate that prior in vivo immunization, which increases the precursor fre-quency, simplifies posterior expansion of tumor- specific CD8+ T cells, and aAPCs is superior to autologous APC for in vitro amplification. This prime and expand regimen can be an alternative method for large amplification of rare tumor-specific CTLs and aAPCs should be a useful tool for ACT immunotherapy.
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등록된 정보가 없습니다.
참고문헌 (17)
참고문헌 신청
/ 1994 / Romagnani S. Th2-like CD8+ T cells showing B cell helper function and reduced cytolytic activity in human immuno-deficiency virus type 1 infection. J Exp Med 1994 :
/ 1994 / immune recognition of self and altered self. J Exp Med 1994 : 1 ~ 4
/ 1994 / transporter associated with antigen processing -indepen-dent presentation of endogenously synthesized peptides is enhanced by endoplasmic reticulum insertion sequenc
/ 1997 / Artificial cell surface constructs for studying receptor-ligand contributions to lymphocyte activation 209 : 47 ~ 57
/ 1998 / A simplified system for generating recombinant adenoviruses 95 : 2509 ~ 2514
/ 1994 / immune recognition of self and altered self. J Exp Med 1994 : 1 ~ 4
/ 1994 / transporter associated with antigen processing -indepen-dent presentation of endogenously synthesized peptides is enhanced by endoplasmic reticulum insertion sequenc
/ 1997 / Artificial cell surface constructs for studying receptor-ligand contributions to lymphocyte activation 209 : 47 ~ 57
/ 1998 / A simplified system for generating recombinant adenoviruses 95 : 2509 ~ 2514
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