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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 811 - 822 (12page)
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초록· 키워드
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Baicalein is one of the major flavonoids in Scutellaria baicalensis Georgi and possesses various effects, including cytoprotection and anti-inflammation. Because endoplasmic reticulum (ER) stress has been implicated in neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, and cerebral ischemia, we investigated the effects of baicalein on apoptotic death of HT22 mouse hippocampal neuronal cells induced by thapsigargin (TG) and brefeldin A (BFA), two representative ER stress inducers. Apoptosis,reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were measured by flow cytometry. Expression level and phosphorylation status of ER stress-associated proteins and activation and cleavage of apoptosis-associated proteins were analyzed by Western blot. Baicalein reduced TG- and BFA-induced apoptosis of HT22 cells and activation and cleavage of apoptosis-associated proteins, such as caspase-12 and -3 and poly(ADP-ribose)polymerase. Baicalein also reduced the TG- and BFA-induced expression of ER stress-associated proteins,including C/EBP homologous protein (CHOP)and glucose-regulated protein 78, the cleavage of X-box binding protein-1 and activating transcription factor 6α, and the phosphorylation of eukaryotic initiation factor-2α and mitogen-activated protein kinases,such as p38, JNK, and ERK. Knock-down of CHOP expression by siRNA transfection and specific inhibitors of p38 (SB203580), JNK (SP600125), and ERK (PD98059) as well as anti-oxidant (N-acetylcysteine)reduced TG- or BFA-induced cell death.
Baicalein also reduced TG- and BFA-induced ROS accumulation and MMP reduction. Taken together, these results suggest that baicalein could protect HT22 neuronal cells against ER stress-induced apoptosis by reducing CHOP induction as well as ROS accumulation and mitochondrial damage.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Arai K / 2004 / Involvement of ERK MAP kinase in endoplasmic reticulum stress in SH-SY5Y human neuroblastoma cells / J Neurochem 89 : 232 ~ 239
Arduino DM / 2009 / Endoplasmic reticulum and mitochondria interplay mediates apoptotic cell death: relevance to Parkinson’s disease / Neurochem Int 55 : 341 ~ 348
Boyce M / 2005 / A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress / Science 307 : 935 ~ 939
Boyce M / 2006 / Cellular response to endoplasmic reticulum stress: a matter of life or death / Cell Death Differ 13 : 363 ~ 373
Breckenridge DG / 2003 / Regulation of apoptosis by endoplasmic reticulum pathways / Oncogene 22 : 8608 ~ 8618
Arduino DM / 2009 / Endoplasmic reticulum and mitochondria interplay mediates apoptotic cell death: relevance to Parkinson’s disease / Neurochem Int 55 : 341 ~ 348
Boyce M / 2005 / A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress / Science 307 : 935 ~ 939
Boyce M / 2006 / Cellular response to endoplasmic reticulum stress: a matter of life or death / Cell Death Differ 13 : 363 ~ 373
Breckenridge DG / 2003 / Regulation of apoptosis by endoplasmic reticulum pathways / Oncogene 22 : 8608 ~ 8618
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