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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 221 - 226 (6page)
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초록· 키워드
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To produce human ferritins in yeast, human H-chain and L-chain ferritins were amplified from previously cloned vectors. Each amplified ferritin gene was inserted into the pYES2.1/V5-His-TOPO yeast expression vector under the control of the GAL1 promoter. Western blot analysis of the recombinant yeast cells revealed that H-and L-chain subunits of human ferritin were expressed in Saccharomyces cerevisiae. Atomic absorption spectrometry (AAS) analysis demonstrated that the intracellular content of iron in the ferritin transformant was 1.6 to 1.8-fold higher than that of the control strain. Ferritin transformants could potentially supply iron-fortified nutrients for food and feed.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Beaumont, C. / 1996 / Novel properties of L type polypeptide subunits in mouse ferritin molecules / J. Bio Chem : 7923 ~ 7926
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Ford, G. C. / 1984 / Ferritin: Design and formation of an iron-storage molecule / Philos Trans R Soc Lond B Bio Sci : 551 ~ 565
Guo, J. H. / 1996 / Expression and loading of recombinant heavy and light chain homopolymers of rat liver ferritin / Arch. Biochem. Biophys : 197 ~ 204
Harrison, P. M. / 1996 / The ferritins: Molecular properties, iron storage function and cellular regulation / Biochim Biophys Acta : 161 ~ 203
Bradford, M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Ford, G. C. / 1984 / Ferritin: Design and formation of an iron-storage molecule / Philos Trans R Soc Lond B Bio Sci : 551 ~ 565
Guo, J. H. / 1996 / Expression and loading of recombinant heavy and light chain homopolymers of rat liver ferritin / Arch. Biochem. Biophys : 197 ~ 204
Harrison, P. M. / 1996 / The ferritins: Molecular properties, iron storage function and cellular regulation / Biochim Biophys Acta : 161 ~ 203
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Improved Coexpression and Multiassembly Properties of RecombinantHuman Ferritin Subunits in Escherichia coli
Journal of Microbiology and Biotechnology
2008 .01
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훼리틴 생산 재조합 효모의 철분 결핍성 빈혈 개선 효과
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Purification and Glycosylation Pattern of Human L-Ferritin in Pichia pastoris
Journal of Microbiology and Biotechnology
2004 .01
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미국흰불나방의 Ferritin의 특성과 철 섭식에 따른 변화
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Expression and Purification of Intact and Functional Soybean (Glycine max)Seed Ferritin Complex in Escherichia coli
Journal of Microbiology and Biotechnology
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곤충의 Ferritin
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1995 .01
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Dietary factors associated with high serum ferritin levels in postmenopausal women with the Fifth Korea National Health and Nutrition Examination Survey (KNHANES V), 2010-2012
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돌돔 ferritin H 유전자의 클로닝과 발현 분석
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Functional Assembly of Recombinant Human Ferritin Subunits in Pichia pastoris
Journal of Microbiology and Biotechnology
2007 .01
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미국흰불나방(Hyphantria cunea) 유충 혈림프의 Ferritin 정제
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1996 .01
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The Effects of Visible Light on Iron Release from Ferritin Related to Lipid Peroxidation in the Retina
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
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Severe Hemorrhage Induced Expressions of Ferritin and Heme Oxygenase-1 in Leukocytes
생명과학회지
2009 .07
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Association of serum ferritin level and depression with respect to the body mass index in Korean male adults
Nutrition Research and Practice
2019 .06
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Association of serum ferritin level and depression with respect to the body mass index in Korean male adults
Nutrition Research and Practice
2019 .06
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붉은머리재주나방(Phalera minor)의 유충 혈림프로 부터 Ferritin의 정제와 특성
한국곤충학회지
1995 .01
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Properties of ferritin embedded in PVA nanofibers
한국진공학회 학술발표회초록집
2007 .08
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Spin properties of ferritin within PVA nanofibers
한국진공학회 학술발표회초록집
2008 .02
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저열량 생즙 식이가 성인의 혈청 페리틴 농도에 미치는 영향
한국지역사회생활과학회지
2015 .11
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Proteomic Analysis of Recombinant Saccharomyces cerevisiae upon Iron Deficiency Induced via Human H-Ferritin Production
Journal of Microbiology and Biotechnology
2008 .01
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