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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제3호
- 발행연도
- 2010.1
- 수록면
- 518 - 524 (7page)
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초록· 키워드
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A mannanase gene (man26B) was obtained from a sea bacterium, Paenibacillus sp. BME-14, through the constructed genomic library and inverse PCR. The gene of man26B had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kDa. Man26B possessed two domains,a carbohydrate binding module (CBM) belonging to family 6 and a family 26 catalytic domain (CD) of glycosyl hydrolases, which showed the highest homology to Cel44C of P. polymyxa (60% identity). The optimum pH and temperature for enzymatic activity of Man26B were 4.5and 60oC, respectively. The activity of Man26B was not affected by Mg2+ and Co2+, but was inhibited by Hg2+,Ca2+, Cu2+, Mn2+, K+, Na+, and β-mercaptoethanol, and slightly enhanced by Pb2+ and Zn2+. EDTA did not affect the activity of Man26B, which indicates that it does not require divalent ions to function. Man26B showed a high specific activity for LBG and konjac glucomannan, with Km,Vmax, and kcat values of 3.80 mg/ml, 91.70 μmol/min/mg protein, and 77.08/s, respectively, being observed when LBG was the substrate. Furthermore, deletion of the CBM6 domain increased the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at 80oC and 90oC for 30 min, respectively.
This finding will be useful in industrial applications of Man26B, because of the harsh circumstances associated with such processes.
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참고문헌 (21)
참고문헌 신청
Araki, R / 2006 / Effect of family 22 carbohydrate-binding module on the thermostability of Xyn10B catalytic module from Clostridium stercorarium / Biosci. Biotechnol. Bioche
Bolam, D. N / 1996 / Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues / Bi
Braithwaite, K. L / 1995 / A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa / Biochem. J 305 : 1005 ~ 1010
Cho, K. M / 2006 / A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains / Appl. Microbiol. Biotechnol 73 : 61
Filichkin, S. A / 2004 / A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development / Plant Physiol 134 : 1080 ~ 1087
Bolam, D. N / 1996 / Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues / Bi
Braithwaite, K. L / 1995 / A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa / Biochem. J 305 : 1005 ~ 1010
Cho, K. M / 2006 / A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains / Appl. Microbiol. Biotechnol 73 : 61
Filichkin, S. A / 2004 / A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development / Plant Physiol 134 : 1080 ~ 1087
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