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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제4호
- 발행연도
- 2010.1
- 수록면
- 670 - 677 (8page)
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초록· 키워드
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An alkaline pectate lyase, Bsp165PelA, was purified to homogeneity from the culture broth of alkaliphilic Bacillus sp. N16-5. The enzyme showed a specific activity as high as 1,000 U/mg and had optimum activity at pH 11.5 and 50oC. It was composed of a single polypeptide chain with a molecular mass of 42 kDa deduced from SDS-PAGE, and its isoelectric point was around pH 6.0.
It could efficiently depolymerize polygalacturonate and pectin. Characterization of product formation revealed unsaturated digalacturonate and trigalacturonate as the main products. The pectate lyase gene (pelA) contained an open reading frame (ORF) of 1,089 bp, encoding a 36-amino acids signal peptide and a mature protein of 326amino acids with a calculated molecular mass of 35.943Da. The deduced amino acid sequence from the pelA ORF exhibited significant homology to those of known pectate lyases in polysaccharide lyase family 1. Some conserved active-site amino acids were found in the deduced amino acid sequence of Bsp165PelA. Ca2+ was not required for activity on pectic substrates.
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참고문헌 (22)
참고문헌 신청
Berensmeier, S / 2004 / Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase /
Blanco, P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol. Lett 175 : 1 ~ 9
Bruhlmann, F / 1995 / Purification and characterization of an extracellular pectate lyase from an Amycolata sp / Appl. Environ. Microbiol 61 : 3580 ~ 3585
Cao, J. Z. L / 1992 / Screening of pectinase producer from alkalophilic bacteria and study on its potential application in degumming of ramie / Enzyme Microb. Technol 14 : 10
Hatada, Y / 2001 / Enzymatic properties of the highly thermophilic and alkaline pectate lyase Pel-4B from alkaliphilic Bacillus sp. strain P-4-N and the entire nucleotide and
Blanco, P / 1999 / Production of pectic enzymes in yeasts / FEMS Microbiol. Lett 175 : 1 ~ 9
Bruhlmann, F / 1995 / Purification and characterization of an extracellular pectate lyase from an Amycolata sp / Appl. Environ. Microbiol 61 : 3580 ~ 3585
Cao, J. Z. L / 1992 / Screening of pectinase producer from alkalophilic bacteria and study on its potential application in degumming of ramie / Enzyme Microb. Technol 14 : 10
Hatada, Y / 2001 / Enzymatic properties of the highly thermophilic and alkaline pectate lyase Pel-4B from alkaliphilic Bacillus sp. strain P-4-N and the entire nucleotide and
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