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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 472 - 476 (5page)
이용수
초록· 키워드
오류제보하기
The present study was untaken to assess thecapacity of Bacillus pumilis KHS-1 to grow on chicken flour andto prepare feather digest as fertilizer. To increase keratinolyticactivity, the addition of cysteine (5.0 mM) showed the highestkeratinolytic activity (245 unit) among the reducing agents tested.The production of soluble protein (feather digests) paralleledthe tendency to the production of keratinolytic protease. In thegrowth curve of B. pumilis KHS-1 at 30oC in the feathermedium with 5 mM cysteine, the maximum keratinolytic activityof B. pumilis was about 161 units/ml after 84 h of incubation.The maximum enzyme activities were observed at the latelogarithmic growth phase, and remained thereafter with littlechanges. Using 27-day plant growth assays on carrot andChinese cabbage, feather digests and reference fertilizer werecompared. In terms of the length and the weight of the abovegroundvegetations, feather digests showed the same effect asthat of the fertilizer. Therefore, our investigation shows thatthe feather digests can be used in agriculture.
목차
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참고문헌 (20)
참고문헌 신청
/ 1993 / Keratinolysis by four fungi isolated from the soil andeffluent of a gelatin factory at Jabalpur : 108 ~ 116
/ 1994 / American Society for Testing and Materials Standard Practice for ConductingEarly Seeding Growth Test AmericanSociety for Testing and Materials : 1061 ~ 94 1067
Berwick / 1991 / Production of keratinolyticenzymes in a rotating frame bioreactor : 359 ~ 362
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye biding : 248 ~ 254
Charney / 1949 / The use of azoalbumin as a substrate in the colorimetricdetermination of peptic and tryptic activity : 428 ~ 433
/ 1994 / American Society for Testing and Materials Standard Practice for ConductingEarly Seeding Growth Test AmericanSociety for Testing and Materials : 1061 ~ 94 1067
Berwick / 1991 / Production of keratinolyticenzymes in a rotating frame bioreactor : 359 ~ 362
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye biding : 248 ~ 254
Charney / 1949 / The use of azoalbumin as a substrate in the colorimetricdetermination of peptic and tryptic activity : 428 ~ 433
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