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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제12호
- 발행연도
- 2017.1
- 수록면
- 2,141 - 2,150 (10page)
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This study was conducted to develop a screening method using Colilert-18 and a device for the detection of E. coli from agri-food production environments and fresh vegetables. The specificity and sensitivity of Colilert-18 by temperature (37°C and 44°C) were evaluated with 38 E. coli and 78 non-E. coli strains. The false-positive rate was 3.8% (3/78) and 0% (0/78) at 37°C and 44°C, respectively. The detection limit of E. coli at 37°C at <1.0 log CFU/250 ml was lower than that at 44°C. The efficiency of the developed device, which comprised an incubator equipped with a UV lamp to detect E. coli in the field, was evaluated by measuring the temperature and UV lamp brightness. The difference between the set temperature and actual temperature of the developed device was about 1.0°C. When applying the developed method and device to various samples, including utensils, gloves, irrigation water, seeds, and vegetables, there were no differences in detection rates of E. coli compared with the Korean Food Code method. For sanitary disposal of culture samples after experiments, the sterilization effect of sodium dichloroisocyanurate (NaDCC) tablets was assessed for use as a substitute for an autoclave. The addition of one tablet of NaDCC per 50 ml was sufficient to kill E. coli cultured in Colilert-18. These results show that the developed protocol and device can efficiently detect E. coli from agri-food production environments and vegetables.
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참고문헌 (22)
참고문헌 신청
Abramson A / 2013 / Adapting enzymebased microbial water quality analysis to remote areas in low-income countries / Environ. Sci. Technol 47 : 10494 ~ 10501
Berger CN / 2010 / Fresh fruit and vegetables as vehicles for the transmission of human pathogens / Environ. Microbiol 12 : 2385 ~ 2397
Berger S. / 1991 / Ability of the Colilert method to recover oxidant-stressed Escherichia coli / Lett. Appl. Microbiol 13 : 247 ~ 250
CDC / 2010 / Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food - 10 states, 2009 / MMWR Morb. Mortal. Wkly. Rep 59 : 418
Covert TC / 1992 / Comparing defined-substrate coliform tests for the detection of Escherichia coli in water / J. Am. Water Works Assoc 84 : 98 ~ 104
Berger CN / 2010 / Fresh fruit and vegetables as vehicles for the transmission of human pathogens / Environ. Microbiol 12 : 2385 ~ 2397
Berger S. / 1991 / Ability of the Colilert method to recover oxidant-stressed Escherichia coli / Lett. Appl. Microbiol 13 : 247 ~ 250
CDC / 2010 / Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food - 10 states, 2009 / MMWR Morb. Mortal. Wkly. Rep 59 : 418
Covert TC / 1992 / Comparing defined-substrate coliform tests for the detection of Escherichia coli in water / J. Am. Water Works Assoc 84 : 98 ~ 104
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