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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 265 - 270 (6page)
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초록· 키워드
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Two major endoglucanase genes (cel7B and cel5A) were cloned from Penicillium decumbens 114-2 using the method of modified thermal asymmetric interlaced polymerase chain reaction (TAIL−PCR). The result of Southern blotting suggested that P. decumbens has a single copy of the cel5A gene and a single copy of the cel7B gene in its chromosomal DNA. The expression levels of cel5A and cel7B were determined by means of real-time quantitative PCR, suggesting that the two genes were coordinately expressed, and repressed by glucose and induced by cellulose. Both endoglucanase genes were expressed in Saccharomyces cerevisiae and the recombinant proteins were purified. The recombinant Cel7B and Cel5A were both optimally active at 60oC and pH4.0. The recombinant Cel7B showed more than 8-fold, 30-fold, and 5-fold higher enzyme activities toward carboxymethyl cellulose, barley β-glucan, and PASC, respectively, in comparison with that of Cel5A. However, their activities toward pNPC and Avicel showed minor differences. The results suggested that Cel7B is a strict endoglucanase,whereas Cel5A showed processivity because of its relative higher ability to hydrolyze the crystal cellulose.
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참고문헌 (23)
참고문헌 신청
Aro, N / 2005 / Transcriptional regulation of plant cell wall degradation by filamentous fungi / FEMS Microbiol. Rev 29 : 719 ~ 739
Bedford, M. R / 1995 / Mechanism of action and potential environmental benefits from the use of feed enzymes / Anim. Feed Sci. Technol 53 : 145 ~ 155
Beguin, P / 1994 / The biological degradation of cellulose / FEMS Microbiol. Rev 13 : 25 ~ 58
Bendtsen, J. D / 2004 / Improved prediction of signal peptides: SignalP 3.0 / J. Mol. Biol 340 : 783 ~ 795
Bhat, M. K / 1997 / Cellulose degrading enzymes and their potential industrial applications / Biotechnol. Adv 15 : 583 ~ 620
Bedford, M. R / 1995 / Mechanism of action and potential environmental benefits from the use of feed enzymes / Anim. Feed Sci. Technol 53 : 145 ~ 155
Beguin, P / 1994 / The biological degradation of cellulose / FEMS Microbiol. Rev 13 : 25 ~ 58
Bendtsen, J. D / 2004 / Improved prediction of signal peptides: SignalP 3.0 / J. Mol. Biol 340 : 783 ~ 795
Bhat, M. K / 1997 / Cellulose degrading enzymes and their potential industrial applications / Biotechnol. Adv 15 : 583 ~ 620
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