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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제12호
발행연도
2007.1
수록면
1,922 - 1,929 (8page)

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A simple whole-cell-based sensing system isH. pluvialis usingultraviolet fluorescence spectroscopy. An emission signal at368 nm was used to detect the various kinds of green, green-brown, brown-red, and red H. pluvialis cells. The fluorescenceemission intensities of the cels were highest at 368 nm withan excitation wavelength of 227 nm. An excitation wavelengthof 227 nm was then selected for cell-mas sensing, as theemission fluorescence intensities of the cel suspensions werehighest at this wavelength after subtracting the backgroundgrade water, filtered water, and HPLC-grade water containinga modified Bold’s basal medium (MBBM) were measured andthe diference was les than 1.6 for the selected wavelengths.Moreover, there was no diference in the emision intensity at368 nm among suspensions of the various morphologicalstates of the cells. A calibration curve of the fluorescenceemission intensities and cel mass was obtained with a highcorrelation (R2=0.938) for the various morphological formsof . Accordingly, the proposed method showed nosignificant dependency on the various morphological cellforms, making it applicable for cell-mas measurement. Ahigh correlation was found between the fluorescence emissionintensities and the dry cel weight with a mixture of green,green-brown, brown-red, and red cels. In conclusion, theproposed model can be directly used for cel-mass sensingwithout any pretreatment and has potential use as a noninvasivemethod for the online determination of algal biomass.

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