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자료유형
학술저널
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제5호
발행연도
2005.1
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1,060 - 1,066 (7page)

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Under environmental stress, such as strong iradianceor nitrogen deficiency, unicellular green algae of the genusHaematococus accumulate secondary carotenoids, i.e. astaxanthin,in the cytosol. The induction and regulation of astaxanthinbiosynthesis in microalgae has recently received considerableattention owing to the increasing use of secondary carotenoidsas a source of pigmentation for fish aquacultures, and as apotential drug in cancer prevention as a fre-radical quencher.Accordingly, this study generated expresed sequence tags(ESTs) from a library constructed from astaxanthin-inducedHaematoccus pluvialis. Partial sequences were obtainedfrom the 5' ends of 1,858 individual cDNAs, and then groupedinto 1,025 non-overlapping sequences, among which 708sequences were singletons, while the remainder fel into 317clusters. Approximately 63% of the EST sequences showedsimilarity to previously described sequences in public databases.H. pluvialis was found to consist of a relatively high percentageof genes involved in genetic information processing (15%)and metabolism (1%), whereas a relatively low percentageof sequences was involved in the signal transduction (3%),structure (2%), and environmental information process (3%). Inadition, a relatively large fraction of H. pluvialis sequenceswas classified as genes involved in photosynthesis (9%) andcellular process (9%). Based on this EST analysis, the full-length cDNA sequence for superoxide dismutase (SOD) of H.pluvialis was cloned, and the expression of this gene wasresponse to different culture conditions, indicating the possibleregulation of this gene in H. pluvialis.

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