지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 696 - 703 (8page)
이용수
초록· 키워드
오류제보하기
A gamma-aminobutyric acid (GABA)-producing microorganism was isolated from jeot-gal (anchovy), a Korean fermented seafood. The isolate, A156, produced GABA profusely when incubated in MRS broth with monosodium glutamate (3% (w/v)) at 37°C for 48 h. A156 was identified as Lactobacillus sakei by 16S rRNA gene sequencing. The GABA conversion yield was 86% as determined by GABase enzyme assay. The gadB gene encoding glutamate decarboxylase (GAD) was cloned by PCR. gadC encoding a glutamate/GABA antiporter was located immediately upstream of gadB. The operon structure of gadCB was confirmed by RT-PCR. gadB was overexpressed in Escherichia coli BL21(DE3) and recombinant GAD was purified. The purified GAD was 54.4 kDa in size by SDS-PAGE. Maximum GAD activity was observed at pH 5.0 and 55°C and the activity was dependent on pyridoxal 5’-phosphate. The Km and Vmax of GAD were 0.045 mM and 0.011 mM/min, respectively, when glutamate was used as the substrate.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Fan E / 2012 / Cloning, sequencing and expression of a glutamate decarboxylase gene from the GABA-producing strain Lactobacillus brevis CGMCC1306 / Ann. Microbiol 62 : 689 ~
Fonda ML / 1985 / L-Glutamate decarboxylase from bacteria / Methods Enzymol 113 : 11 ~ 16
Hiraga K / 2008 / Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate / Biosci. Biotechnol. Biochem 72 : 1299 ~ 13065
Ki-Bum Park / 2004 / Cloning and Expression of a Full-Length Glutamate Decarboxylase Gene from Lactobacillus plantarum / Preventive Nutrition and Food Science 9 (4) : 324 ~
Fan E / 2012 / Cloning, sequencing and expression of a glutamate decarboxylase gene from the GABA-producing strain Lactobacillus brevis CGMCC1306 / Ann. Microbiol 62 : 689 ~
Fonda ML / 1985 / L-Glutamate decarboxylase from bacteria / Methods Enzymol 113 : 11 ~ 16
Hiraga K / 2008 / Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate / Biosci. Biotechnol. Biochem 72 : 1299 ~ 13065
Ki-Bum Park / 2004 / Cloning and Expression of a Full-Length Glutamate Decarboxylase Gene from Lactobacillus plantarum / Preventive Nutrition and Food Science 9 (4) : 324 ~
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization of the Recombinant Glutamate Decarboxylase of Lactobacillus brevis G144 Isolated from Galchi Jeotgal, a Korean Salted and Fermented Seafood
한국미생물·생명공학회지
2021 .01
-
Characterization of a Glutamate Decarboxylase (GAD) from Enterococcus avium M5 Isolated from Jeotgal, a Korean Fermented Seafood
Journal of Microbiology and Biotechnology
2017 .07
-
Bioconversion of Gamma-Aminobutyric Acid from Monosodium Glutamate by Lactobacillus brevis Bmb5
Journal of Microbiology and Biotechnology
2019 .01
-
Production of γ-Aminobutyric Acid Using Immobilized Glutamate Decarboxylase from Lactobacillus plantarum
한국미생물·생명공학회지
2015 .01
-
Enchancement of Gamma-Aminobutyric Acid Production by Co- Localization of Neurospora crassa OR74A Glutamate Decarboxylase with Escherichia coli GABA Transporter Via Synthetic Scaffold Complex
Journal of Microbiology and Biotechnology
2017 .01
-
한국전통주인 막걸리로부터 분리한 Lactobacillus plantarum subsp. plantarum B-134의 gamma-aminobutyric acid (GABA)의 생산
생명과학회지
2017 .05
-
Enhanced Production of Gamma-Aminobutyric Acid by Optimizing Culture Conditions of Lactobacillus brevis HYE1 Isolated from Kimchi, a Korean Fermented Food
Journal of Microbiology and Biotechnology
2017 .01
-
Co-Localization of GABA Shunt Enzymes for the Efficient Production of Gamma-Aminobutyric Acid via GABA Shunt Pathway in Escherichia coli
Journal of Microbiology and Biotechnology
2016 .01
-
Globular Adiponectin Exerts a Pro-Inflammatory Effect via IκB/NF-κB Pathway Activation and Anti-Inflammatory Effect by IRAK-1 Downregulation
Molecules and Cells
2018 .08
-
Optimization of γ-Aminobutyric Acid Production by Enterococcus faecium JK29 Isolated from a Traditional Fermented Foods
한국미생물·생명공학회지
2016 .01
-
Gamma-Aminobutyric Acid Production from a Novel Enterococcus avium JS-N6B4 Strain Isolated from Edible Insects
Journal of Microbiology and Biotechnology
2019 .01
-
Characteristics of Potential Gamma-Aminobutyric Acid-Producing Bacteria Isolated from Korean and Vietnamese Fermented Fish Products
Journal of Microbiology and Biotechnology
2019 .01
-
Identification, Expression and Preliminary Characterization of a Recombinant Bifunctional Enzyme of Photobacterium damselae subsp. piscicida with Glutamate Decarboxylase/Transaminase Activity
한국미생물·생명공학회지
2019 .01
-
Characterization of the Production of Biogenic Amines and Gamma-Aminobutyric Acid in the Soybean Pastes Fermented by Aspergillus oryzae and Lactobacillus brevis
Journal of Microbiology and Biotechnology
2015 .01
-
γ-Aminobutyric Acid (GABA) Production and Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity of Fermented Soybean Containing Sea Tangle by the Co-Culture of Lactobacillus brevis with Aspergillus oryzae
Journal of Microbiology and Biotechnology
2015 .01
-
The Role of Glutamic Acid-producing Microorganisms in Rumen Microbial Ecosystems
생명과학회지
2021 .05
-
Acceleration of Aglycone Isoflavone and γ-Aminobutyric Acid Production from Doenjang Using Whole-Cell Biocatalysis Accompanied by Protease Treatment
Journal of Microbiology and Biotechnology
2017 .01
-
Intracellular Flux Prediction of Recombinant Escherichia coli Producing Gamma-Aminobutyric Acid
Journal of Microbiology and Biotechnology
2024 .04
-
Characterization and Immunomodulation Activity of Lactobacillus sakei L2 and L8 Isolated from Chicken Cecum
한국미생물·생명공학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0