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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제7호
- 발행연도
- 2018.1
- 수록면
- 1,141 - 1,146 (6page)
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초록· 키워드
오류제보하기
2’-Fucosyllactose (2’-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2’-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2’-FL in a microplate format. The method involves two steps: (i) release of L-fucose from 2’-FL by α-(1-2,3,4,6)-L-fucosidase and (ii) measurement of NADPH formed during the oxidation of L-fucose by L-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2’-FL in 50 min using a 96- well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.
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참고문헌 (23)
참고문헌 신청
Petschacher B / 2016 / Biotechnological production of fucosylated human milk oligosaccharides: prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems / J. Biotechnol. 235:61~83
Castanys-Munoz E / 2013 / 2'- Fucosyllactose: an abundant, genetically determined soluble glycan present in human milk / Nutr. Rev. 71:773~789
Barile D / 2013 / Human milk and related oligosaccharides as prebiotics / Curr. Opin. Biotechnol. 24:214~219
Morrow AL / 2004 / Human milk oligosaccharides are associated with protection against diarrhea in breast-fed infants / J. Pediatr. 145:297~303
Newburg DS / 2005 / Human milk glycans protect infants against enteric pathogens / Annu. Rev. Nutr. 25:37~58
Castanys-Munoz E / 2013 / 2'- Fucosyllactose: an abundant, genetically determined soluble glycan present in human milk / Nutr. Rev. 71:773~789
Barile D / 2013 / Human milk and related oligosaccharides as prebiotics / Curr. Opin. Biotechnol. 24:214~219
Morrow AL / 2004 / Human milk oligosaccharides are associated with protection against diarrhea in breast-fed infants / J. Pediatr. 145:297~303
Newburg DS / 2005 / Human milk glycans protect infants against enteric pathogens / Annu. Rev. Nutr. 25:37~58
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