지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 64 - 70 (7page)
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초록· 키워드
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Dirofilaria immitis (D. immitis) is a filarial nematode that causes cardiopulmonary dirofilariasis in dogs. In the late stages of infection, infected dogs show one or more symptoms and advanced heart disorder with perivascular inflammation. To detect D. immitis specifically and efficiently in the early stages of infection, a duplex TaqMan qPCR assay was developed based on previous studies using primers and probes specialized to detect D. immitis cytochrome c oxidase subunit I (COI ) and dog glyceraldehyde-3-phosphate dehydrogenase (GAPDH). As positive controls, plasmid DNAs were constructed from D. immitis COI or dog GAPDH and a TA-cloning vector. Simplex and duplex TaqMan qPCR assays were performed using the specific primers, probes, and genomic or plasmid DNA. The duplex reaction developed could detect D. immitis COI and dog GAPDH in the same sample simultaneously after optimization of the primer concentrations. The limit of detection was 25 copies for the simplex and duplex assays, and both showed good linearity, high sensitivity, and excellent PCR efficiency. The duplex assays for pathogen detection reduce the costs, labor, and time compared to simplex reactions. Therefore, the duplex TaqMan qPCR assay developed herein will allow efficient D. immitis detection and quantification from a large number of samples simultaneously.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Watts KJ / 1999 / Development of a PCR-and probe-based test for the sensitive and specific detection of the dog heartworm, Dirofilaria immitis, in its mosquito intermediate host / Mol Cell Probes 13:425~430
Kronefeld M / 2014 / Molecular detection of Dirofilaria immitis, Dirofilaria repens and Setaria tundra in mosquitoes from Germany / Parasit Vectors 7:30
Rojas A / 2015 / Detection of Dirofilaria immitis and other arthropod-borne filarioids by an HRM real-time qPCR, blood-concentrating techniques and a serological assay in dogs from Costa Rica / Parasit Vectors 8:170
Xu D / 2015 / Molecular detection of vector-borne agents in dogs from ten provinces of China / Parasit Vectors 8:501
Oi M / 2015 / Detection of Dirofilaria immitis DNA in host serum by nested PCR / Parasitol Res 114:3645~3648
Kronefeld M / 2014 / Molecular detection of Dirofilaria immitis, Dirofilaria repens and Setaria tundra in mosquitoes from Germany / Parasit Vectors 7:30
Rojas A / 2015 / Detection of Dirofilaria immitis and other arthropod-borne filarioids by an HRM real-time qPCR, blood-concentrating techniques and a serological assay in dogs from Costa Rica / Parasit Vectors 8:170
Xu D / 2015 / Molecular detection of vector-borne agents in dogs from ten provinces of China / Parasit Vectors 8:501
Oi M / 2015 / Detection of Dirofilaria immitis DNA in host serum by nested PCR / Parasitol Res 114:3645~3648
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