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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제7호
- 발행연도
- 2019.1
- 수록면
- 1,043 - 1,052 (10page)
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Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with p-NPC16 at a pH of 8.5 and 50°C, and the Km, kcat, and kcat/Km values were 1.05 mM, 292.95 s-1 and 279 s-1mM-1, respectively. The lipase was highly stable at 7.5 ≤ pH ≤ 10.0. K+ and Na+ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.
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참고문헌 (40)
참고문헌 신청
Contesini FJ / 2010 / Aspergillus sp, lipase: potential biocatalyst for industrial use / J. Mol. Catal. B-Enzym 67:163~171
Shu Z Y / 2007 / Resources and current state of lipases used in biodiesel production / Hubei Agric. Sci 6:64
Fojan P / 2000 / What distinguishes an esterase from a lipase : A novel structural approach / Biochimie 82:1033~1041
Winkler FK / 1990 / Structure of human pancreatic lipase / Nature 343:771~774
Zheng YY / 2011 / Thermophilic lipase from Thermomyces lanuginosus : Gene cloning, expression and characterization / J. Mol. Catal. B Enzym 69:127~132
Shu Z Y / 2007 / Resources and current state of lipases used in biodiesel production / Hubei Agric. Sci 6:64
Fojan P / 2000 / What distinguishes an esterase from a lipase : A novel structural approach / Biochimie 82:1033~1041
Winkler FK / 1990 / Structure of human pancreatic lipase / Nature 343:771~774
Zheng YY / 2011 / Thermophilic lipase from Thermomyces lanuginosus : Gene cloning, expression and characterization / J. Mol. Catal. B Enzym 69:127~132
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