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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제2호
- 발행연도
- 2020.1
- 수록면
- 196 - 205 (10page)
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In this study, the acidic lipase from Aspergillus niger (ANL) was homologously expressed in A. niger. The expression of ANL was significantly improved by the expression of the native ANL with the introns, the addition of the Kozak sequence and the optimization of the signal sequences. When the cDNA sequence of ANL fused with the glaA signal was expressed under the gpdA promoter in A. niger, no lipase activity could be detected. We then tried to improve the expression by using the full-length ANL gene containing three introns, and the lipase activity in the supernatant reached 75.80 U/ml, probably as a result of a more stable mRNA structure. The expression was further improved to 100.60 U/ml by introducing a Kozak sequence around the start codon due to a higher translation efficiency. Finally, the effects of three signal sequences including the cbhI signal, the ANL signal and the glaA signal on the lipase expression were evaluated. The transformant with the cbhI signal showed the highest lipase activity (314.67 U/ml), which was 1.90-fold and 3.13-fold higher than those with the ANL signal and the glaA signal, respectively. The acidic lipase was characterized and its highest activity was detected at pH 3.0 and a temperature of 45ºC. These results provided promising strategies for the production of the acidic lipase from A. niger.
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참고문헌 (39)
참고문헌 신청
Schuster E / 2002 / On the safety of Aspergillus niger-A review / Appl. Microbiol. Biotechnol 59:426~435
Cairns TC / 2018 / How a fungus shapes biotechnology : 100 years of Aspergillus niger research / Fungal Biol. Biotechnol 5:13
Steiniger C / 2017 / Harnessing fungal nonribosomal cyclodepsipeptide synthetases for mechanistic insights and tailored engineering / Chem. Sci 8:7834~7843
Michael W / 2004 / Characterization of humanized antibodies secreted by Aspergillus niger / Appl. Environ. Microbiol 70:2567~2576
Punt PJ / 2002 / Filamentous fungi as cell factories for protein production / Trends Biotechnol 20:200~206
Cairns TC / 2018 / How a fungus shapes biotechnology : 100 years of Aspergillus niger research / Fungal Biol. Biotechnol 5:13
Steiniger C / 2017 / Harnessing fungal nonribosomal cyclodepsipeptide synthetases for mechanistic insights and tailored engineering / Chem. Sci 8:7834~7843
Michael W / 2004 / Characterization of humanized antibodies secreted by Aspergillus niger / Appl. Environ. Microbiol 70:2567~2576
Punt PJ / 2002 / Filamentous fungi as cell factories for protein production / Trends Biotechnol 20:200~206
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