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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 478 - 487 (10page)
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초록· 키워드
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Background: Plasma epidermal growth factor receptor (EGFR) mutation tests are less invasive than tissue EGFR mutation tests. We determined which of two kits is more efficient: cobas EGFR Mutation test v2 (cobasv2; Roche Molecular Systems, Pleasanton, CA, USA) or PANAMutyper-R-EGFR (Mutyper; Panagene, Daejeon, Korea). We also evaluated whether pleural effusion supernatant (PE-SUP) samples are assayable, similar to plasma samples, using these two kits. Methods: We analyzed 156 plasma and PE-SUP samples (31 paired samples) from 116 individuals. We compared the kits in terms of accuracy, assessed genotype concordance (weighted κ with 95% confidence intervals), and calculated Spearman’s rho between semi-quantitatively measured EGFR-mutant levels (SQIs) measured by each kit. We also compared sensitivity using 47 EGFR-mutant harboring samples divided into more-dilute and less-dilute samples (dilution ratio: ≥ or <1:1,000). Results: cobasv2 tended to have higher accuracy than Mutyper (73% vs 69%, P=0.53), and PE-SUP samples had significantly higher accuracy than plasma samples (97% vs 55–71%) for both kits. Genotype concordance was 98% (κ=0.92, 0.88–0.96). SQIs showed strong positive correlations (P<0.0001). In less-dilute samples, accuracy and sensitivity did not differ significantly between kits. In more-dilute samples, cobasv2 tended to have higher sensitivity than Mutyper (43% vs 20%, P=0.07). Conclusions: The kits have similar performance in terms of EGFR mutation detection and semi-quantification in plasma and PE-SUP samples. cobasv2 tends to outperform Mutyper in detecting less-abundant EGFR-mutants. PE-SUP samples are assayable using either kit.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Sharma SV / 2007 / Epidermal growth factor receptor mutations in lung cancer / Nat Rev Cancer 7:169~181
Lee CK / 2013 / Impact of EGFR inhibitor in non-small cell lung cancer on progression-free and overall survival : a meta-analysis / J Natl Cancer Inst 105:595~605
Yu HA / 2013 / Analysis of tumor specimens at the time of acquired resistance to EGFR-TKI therapy in 155 patients with EGFR-mutant lung cancers / Clin Cancer Res 19:2240~2247
Tan DS / 2016 / The International Association for the Study of Lung Cancer consensus statement on optimizing management of EGFR mutation-positive non-small cell lung cancer : status in 2016 / J Thorac Oncol 11:946~963
Mok TS / 2017 / Osimertinib or platinum-pemetrexed in EGFR T790M-positive lung cancer / N Engl J Med 376:629~640
Lee CK / 2013 / Impact of EGFR inhibitor in non-small cell lung cancer on progression-free and overall survival : a meta-analysis / J Natl Cancer Inst 105:595~605
Yu HA / 2013 / Analysis of tumor specimens at the time of acquired resistance to EGFR-TKI therapy in 155 patients with EGFR-mutant lung cancers / Clin Cancer Res 19:2240~2247
Tan DS / 2016 / The International Association for the Study of Lung Cancer consensus statement on optimizing management of EGFR mutation-positive non-small cell lung cancer : status in 2016 / J Thorac Oncol 11:946~963
Mok TS / 2017 / Osimertinib or platinum-pemetrexed in EGFR T790M-positive lung cancer / N Engl J Med 376:629~640
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